Brain, Vol 120, Issue 8 1447-1460, Copyright © 1997 by Oxford University Press
JM Greer, PA Csurhes, KD Cameron, PA McCombe, MF Good and MP Pender
We tested the proliferative responses of peripheral blood mononuclear cells
from 61 patients with multiple sclerosis, 56 healthy control subjects and
52 patients with other neurological diseases to seven synthetic peptides of
myelin proteolipid protein (PLP) and 19 synthetic peptides of myelin basic
protein (MBP). Increased proliferative responses to two overlapping PLP
peptides, PLP184-199 and PLP190-209 were found significantly more
frequently in blood from patients with relapsing-remitting or secondary
progressive multiple sclerosis (52.3%), but not from those with primary
progressive multiple sclerosis (18.2%), than in that from healthy control
subjects (8.9%) and patients with other neurological diseases (20.8%).
Reactivity to these PLP peptides was most frequently seen in blood from
patients with multiple sclerosis of 6-15 years duration and with moderate
to severe disability (Kurtzke's Expanded Disability Status Scale > 4.0);
the blood from 15 of 19 patients in this group reacted to one or both of
the peptides. Both peptides could be recognized by short-term T-cell lines
specific for whole PLP, and lines specific for one or other of the two
overlapping peptides were able to recognize whole PLP, indicating that
these peptides can be processed naturally from the intact molecule. This
region of PLP is encephalitogenic in a number of strains of mice. Samples
from multiple sclerosis patients did not react more frequently to any of
the MBP peptides than those from healthy control subjects. The proportions
of patients with other neurological diseases whose blood responded to the
MBP peptides that most frequently elicited responses in blood from multiple
sclerosis patients were significantly lower than the proportions of
multiple sclerosis patients and healthy control subjects whose blood
responded to these peptides.
ARTICLES
Increased immunoreactivity to two overlapping peptides of myelin proteolipid protein in multiple sclerosis
Department of Medicine, University of Queensland, Australia.
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