Brain, Vol 121, Issue 6 1073-1087, Copyright © 1998 by Oxford University Press
R Kohling, A Lucke, H Straub, EJ Speckmann, I Tuxhorn, P Wolf, H Pannek and F Oppel
Human neocortical temporal lobe tissue resected for treatment of
pharmacoresistant epilepsy was investigated. In slices prepared from this
tissue, field potentials sometimes superimposed by population spikes were
found to appear spontaneously. In individual slices, they were generalized
or highly localized to a field of approximately 200 microns in diameter.
Synchronous with these potentials, hyperpolarizing and depolarizing
postsynaptic potentials were recorded from neurons in the vicinity of the
field potential electrode. Hyperpolarizing postsynaptic potentials appeared
to be mainly chloride mediated. All potentials, i.e. sharp field potentials
as well as postsynaptic potentials, were reversibly suppressed by blockade
of the non-NMDA (non- N-methyl-D-aspartate) glutamate-subreceptor and of
the GABAA (gamma- aminobutyric acid) receptor, and by application of the
organic calcium channel blocker verapamil. By contrast, all potentials
remained unaffected by blockade of the NMDA glutamate-subreceptor and the
GABAB receptor. The antiepileptic drugs carbamazepine and phenytoin failed
to suppress the spontaneous potentials at therapeutic concentrations.
Washout of Mg2+ from the superfusate left the spontaneous potentials
unchanged or converted them to ictal-type discharges. This epileptiform
activity was not suppressed, but augmented by blockade of the GABAA
receptor. As a whole, the spontaneously appearing field potentials may be
assumed to reflect a state of increased neuronal synchronization.
ARTICLES
Spontaneous sharp waves in human neocortical slices excised from epileptic patients
Institut fur Physiologie, Munster, Germany.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. P. Wu, H. L. Huang, M. N. Asl, J. W. He, J. Gillis, F. K. Skinner, and L. Zhang Spontaneous rhythmic field potentials of isolated mouse hippocampal-subicular-entorhinal cortices in vitro J. Physiol., October 15, 2006; 576(2): 457 - 476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. Hekmat-Scafe, M. Y. Lundy, R. Ranga, and M. A. Tanouye Mutations in the K+/Cl- Cotransporter Gene kazachoc (kcc) Increase Seizure Susceptibility in Drosophila. J. Neurosci., August 30, 2006; 26(35): 8943 - 8954. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. D. Micheva, C. P. Taylor, and S. J Smith Pregabalin Reduces the Release of Synaptic Vesicles from Cultured Hippocampal Neurons Mol. Pharmacol., August 1, 2006; 70(2): 467 - 476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. T. Kantrowitz, N. N. Francis, A. Salah, and K. L. Perkins Synaptic Depolarizing GABA Response in Adults Is Excitatory and Proconvulsive When GABAB Receptors Are Blocked J Neurophysiol, May 1, 2005; 93(5): 2656 - 2667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Wu, W. P. Luk, J. Gillis, F. Skinner, and L. Zhang Size Does Matter: Generation of Intrinsic Network Rhythms in Thick Mouse Hippocampal Slices J Neurophysiol, April 1, 2005; 93(4): 2302 - 2317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. G. Jones and G. L. Woodhall Background synaptic activity in rat entorhinal cortical neurones: differential control of transmitter release by presynaptic receptors J. Physiol., January 1, 2005; 562(1): 107 - 120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Hausser, I. M. Raman, T. Otis, S. L. Smith, A. Nelson, S. du Lac, Y. Loewenstein, S. Mahon, C. Pennartz, I. Cohen, et al. The Beat Goes On: Spontaneous Firing in Mammalian Neuronal Microcircuits J. Neurosci., October 20, 2004; 24(42): 9215 - 9219. [Full Text] [PDF]
|
|
|
|
 |
|
 E. Palma, D. A. Ragozzino, S. Di Angelantonio, G. Spinelli, F. Trettel, A. Martinez-Torres, G. Torchia, A. Arcella, G. Di Gennaro, P. P. Quarato, et al. Phosphatase inhibitors remove the run-down of {gamma}-aminobutyric acid type A receptors in the human epileptic brain PNAS, July 6, 2004; 101(27): 10183 - 10188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Rivera, J. Voipio, J. Thomas-Crusells, H. Li, Z. Emri, S. Sipila, J. A. Payne, L. Minichiello, M. Saarma, and K. Kaila Mechanism of Activity-Dependent Downregulation of the Neuron-Specific K-Cl Cotransporter KCC2 J. Neurosci., May 12, 2004; 24(19): 4683 - 4691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Maier, V. Nimmrich, and A. Draguhn Cellular and Network Mechanisms Underlying Spontaneous Sharp Wave-Ripple Complexes in Mouse Hippocampal Slices J. Physiol., August 1, 2003; 550(3): 873 - 887. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Cohen, V. Navarro, S. Clemenceau, M. Baulac, and R. Miles On the Origin of Interictal Activity in Human Temporal Lobe Epilepsy in Vitro Science, November 15, 2002; 298(5597): 1418 - 1421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Maier, M. Guldenagel, G. Sohl, H. Siegmund, K. Willecke, and A. Draguhn Reduction of high-frequency network oscillations (ripples) and pathological network discharges in hippocampal slices from connexin 36-deficient mice J. Physiol., June 1, 2002; 541(2): 521 - 528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Gabel and J. J. LoTurco Layer I Ectopias and Increased Excitability in Murine Neocortex J Neurophysiol, May 1, 2002; 87(5): 2471 - 2479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kohling, J.-M. Hohling, H. Straub, D. Kuhlmann, U. Kuhnt, I. Tuxhorn, A. Ebner, P. Wolf, H.-W. Pannek, A. Gorji, et al. Optical Monitoring of Neuronal Activity During Spontaneous Sharp Waves in Chronically Epileptic Human Neocortical Tissue J Neurophysiol, October 1, 2000; 84(4): 2161 - 2165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Kohling, M. Vreugdenhil, E. Bracci, and J. G. R. Jefferys Ictal Epileptiform Activity Is Facilitated by Hippocampal GABAA Receptor-Mediated Oscillations J. Neurosci., September 15, 2000; 20(18): 6820 - 6829. [Abstract] [Full Text] [PDF]
|
|