Brain, Vol. 122, No. 3, 523-535,
March 1999
© 1999 Oxford University Press
Article |
Heterogeneity of T-cell receptor usage in experimental autoimmune neuritis in the Lewis rat
.
1 Departments of Neurology and 2 Virology and Immunobiology, Julius-Maximilians Universität, Würzburg, Germany and 3 Immunology Department/IMM3 Scripps Clinic and Research Foundation, La Jolla, California, USA
Correspondence to:
Ralf Gold, MD, Department of Neurology, University of Würzburg, Josef-Schneider-Straße 11, D-97080 Würzburg, Germany E-mail r.gold{at}mail.uni-wuerzburg.de
In experimental autoimmune neuritis (EAN), T-cell receptor (TCR) variable (V)-region gene usage by neuritogenic T cells has been reported to be clonally restricted at the RNA level. This study was designed to verify TCR usage by neuritogenic T cells at the protein level. We generated two monoclonal antibodies (mAbs) 7H4 and 8G8 specific for a Vß4/V
11 associated idiotype expressed by the majority of neuritogenic cells of P2-specific T-cell lines. The remaining neuritogenic P2-specific T cells either exhibited a dominant usage of the TCR Vß13 chain recognized by the recently generated mAbs 17D5 and 18B1 or showed diverse Vß usage. Treatment of adoptive-transfer (AT)-EAN or of EAN actively induced with the neuritogenic P2 peptide by mAbs 7H4 and 8G8 led to a partial, but significant, reduction of clinical disease. Treatment with Vß13-specific mAb 17D5 had no clear effect on active EAN. Our data show that at least three different TCR are used by P2-specific pathogenic T cells in EAN, an animal model for human inflammatory neuropathies.
Guillain–Barré syndrome; EAN therapy; antibodies; T-cell receptors
AT-EAN = adoptive transfer EAN; cDNA = complementary DNA; EAE = experimental autoimmune encephalomyelitis; EAN = experimental autoimmune neuritis; FACS = fluorescence-activated cell sorter; mAb = monoclonal antibody; MBP = myelin basic protein; PCR = polymerase chain reaction; rhP2 = recombinant human P2; RT–PCR = reverse transcription–PCR; TCR = T-cell receptor; V = variable
* Present address: Department of Neurology, University of Regensburg, 93053, Regensburg, Germany
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. A. Haque, T. Mizobuchi, K. Yasufuku, T. Fujisawa, R. R. Brutkiewicz, Y. Zheng, K. Woods, G. N. Smith, O. W. Cummings, K. M. Heidler, et al. Evidence for Immune Responses to a Self-Antigen in Lung Transplantation: Role of Type V Collagen-Specific T Cells in the Pathogenesis of Lung Allograft Rejection J. Immunol., August 1, 2002; 169(3): 1542 - 1549. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Stienekemeier, K. Falk, O. Rotzschke, A. Weishaupt, C. Schneider, K. V. Toyka, R. Gold, and J. L. Strominger Vaccination, prevention, and treatment of experimental autoimmune neuritis (EAN) by an oligomerized T cell epitope PNAS, November 20, 2001; 98(24): 13872 - 13877. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Schmid, M. Stienekemeier, S. Oehen, F. Bootz, J. Zielasek, R. Gold, K. V. Toyka, M. Schachner, and R. Martini Immune Deficiency in Mouse Models for Inherited Peripheral Neuropathies Leads to Improved Myelin Maintenance J. Neurosci., January 15, 2000; 20(2): 729 - 735. [Abstract] [Full Text] [PDF]
|
|