| ||||||||||||||||||||||
Fig. 5 Persistent expansion of T cell clone A6 and relationship with CD8+ T cell recognition of HTLV-I Tax11-19/A2 complex in a patient with HAM/TSP. (A) Stable frequency of A6 in the peripheral blood over several years of chronic progression of HAM/TSP. (B) Increased frequency of A6 in sorted CD8+ cells and in unmanipulated CSF cells (12-fold). (C) Staining of PBMC from a healthy control (left) and patient HAM3 (right) with A2 Ig loaded with HTLV-I Tax11-19 (top) or with a control peptide, HIV gag77-85 (bottom). Percentages in upper right quadrants indicate the proportion of Tax/A2 Ig positive CD8+ cells in the total CD8+ cells. (D) By staining with Tax/A2 Ig and TCCT, we determined that clone A6 alone represents 14.2% (or, one of seven) of all Tax11-19-specific, A2-restricted CD8+ T cells in the peripheral blood.
| ||||||||||||||||||||||