Brain Advance Access originally published online on May 3, 2006
Brain 2006 129(7):1659-1673; doi:10.1093/brain/awl082
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Review Articles |
Plasticity in the human central nervous system
Division of Neurophysiology, National Institute for Medical Research London, UK
Correspondence to: Dr Sam Cooke, Division of Neurophysiology, National Institute for Medical Research, Mill Hill, London NW7 1AA, UK E-mail: scooke{at}nimr.mrc.ac.uk
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Summary |
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Summary
IntroductionLong-term potentiation (LTP) is a well-characterized form of synaptic plasticity that fulfils many of the criteria for a neural correlate of memory. LTP has been studied in a variety of animal models and, in rodents in particular, there is now a strong body of evidence demonstrating common underlying molecular mechanisms in LTP and memory. Results are beginning to emerge from studies of neural plasticity in humans. This review will summarize findings demonstrating that synaptic LTP can be induced in human CNS tissue and that rodent and human LTP probably share similar molecular mechanisms. We will also discuss the application of non-invasive stimulation techniques to awake human subjects to induce LTP-like long-lasting changes in localized neural activity. These techniques have potential therapeutic application in manipulating neural plasticity to treat a variety of conditions, including depression, Parkinson's disease, epilepsy and neuropathic pain.
Key Words: long-term potentiation; long-term depression; transcranial magnetic stimulation; interventional paired associative stimulation; NMDA receptor
Abbreviations:
AMPA, 
-amino-3-hydroxy-5-methylisoxazole-propionate; CA1, cornus ammonis 1; CaMKII, calcium/calmodulin-dependent kinase II; cAMP, cyclic adenosine monophosphate; CREB, cAMP-responsive element binding protein; ERP, event-related potential; IPAS, interventional paired associative stimulation; LTD, long-term depression; LTP, long-term potentiation; MPTP, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; NMDA, N-methyl-D-aspartate; NR1, 2A and 2B, NMDA receptor subunits 1, 2A and 2B; PKA, cAMP-dependent protein kinase; rTMS, repetitive transcranial magnetic stimulation.
Received December 19, 2005. Revised March 7, 2006. Accepted March 9, 2006.
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Introduction |
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Long-term potentiation (LTP) of chemical synaptic transmission and the converse process of long-term depression (LTD) are the most widely studied physiological models of memory formation in the mammalian brain. LTP results from coincident activity of pre- and post-synaptic elements, bringing about a facilitation of chemical transmission that lasts for hours in vitro, and that can persist for periods of weeks or months in vivo (Bliss and Gardner-Medwin, 1973
; Abraham et al., 2002). LTP was initially demonstrated at glutamatergic synapses between medial perforant path fibres, emanating from the entorhinal cortex, and granule cells in the dentate gyrus of the hippocampus of the anaesthetized rabbit (Bliss and Lømo, 1973). Subsequently, LTP has been studied in a variety of species, ranging from mice (Nosten-Bertrand et al., 1996) to monkeys (Urban et al., 1996), and at a number of different synapses throughout the central nervous system (CNS), from the cerebral neocortex (Fox, 2002) to the spinal cord (Ji et al., 2003). In this article, we show that there is now a convincing body of evidence to suggest that LTP and other forms of neural plasticity can occur in the human CNS. We also discuss the potential clinical applications of manipulating neural plasticity through non-invasive stimulation methods in humans. To begin with, we provide a brief review of the animal literature that has informed our current understanding of the basic features and molecular mechanisms underlying LTP.
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Long-term potentiation |
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Early experiments on anaesthetized animals used extracellular electrodes to monitor synchronous, synaptically evoked responses of large populations of cells in the tightly delineated layers of the hippocampus—typically in the dentate gyrus. The introduction of the hippocampal slice preparation (Skrede and Westgaard, 1971
), in which 400–500 µm thick transverse slices are kept alive for several hours in an oxygenated bath of artificial cerebrospinal fluid, has enabled easy access to the cornus ammonis cornus ammonis 3 (CA3) and cornus ammonis 1 (CA1) pyramidal subfields. The placement of recording and stimulating electrodes can be easily determined by eye in this preparation, drugs can be added to and washed out of the bathing medium and stable recordings of evoked potentials can be collected over many hours. It is for these reasons that the majority of work on LTP has been conducted in vitro, usually in the CA1 subfield of the hippocampus. From the beginning, experimenters have used high-frequency trains of electrical stimuli (tetani), delivered to Schaffer collateral/commissural fibres projecting from CA3 to CA1 pyramidal neurons, thereby ensuring sufficient synaptic input to induce action potentials post-synaptically. In later experiments, intracellular techniques were used to pair depolarization of a post-synaptic cell with simultaneous afferent stimulation, providing a demonstration at the single-cell level that coincidence between pre- and post-synaptic activity is essential for LTP induction (Gustafsson et al., 1987; Markram et al., 1997; Bi and Poo, 1998).
In addition to its longevity, LTP has other characteristics that make it an attractive candidate mechanism for the storage of information, characteristics that were predicted by the neuropsychologist Donald Hebb long before the discovery of LTP (Hebb, 1949). First, synaptic LTP is an input-specific process, such that a single pathway can be potentiated without effect on inactive neighbouring inputs to the same cell (Andersen et al., 1980; Barrionuevo and Brown, 1983). Since cortical neurons can receive thousands of synaptic inputs, this feature could greatly enlarge the information encoding capacity of the brain; if pathway-specificity in fact reflects synapse-specificity, then the unit of information storage could be a single synapse. Second, the property of associativity ensures that a weak tetanus, which is not by itself capable of initiating LTP, can become potentiated through association with a strong tetanus (McNaughton et al., 1978; Levy and Steward, 1979). This latter mechanism is of particular interest in relation to memory formation as it presents a means by which we can associate events or entities in the outside world—a defining feature of much animal learning from classical and operant conditioning up to higher-order cognitive processing.
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Molecular mechanisms |
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The property of associativity relies upon a mechanism that detects coincident pre- and post-synaptic activity. At most glutamatergic synapses in the CNS the N-methyl-D-aspartate (NMDA) sub-class of glutamate receptor performs this function (Collingridge et al., 1983
) (see Fig. 1). Post-synaptically positioned NMDA receptors bind glutamate released into the synaptic cleft following the invasion of the pre-synaptic terminal by an afferent action potential. This event alone does not open the NMDA receptor's intrinsic cation channel, however, since at near-resting membrane potentials the channel is blocked by magnesium ions (Nowak et al., 1984). Only when the post-synaptic cell is sufficiently depolarized is the magnesium ion expelled from the cation channel, allowing an influx of sodium and calcium ions into the cell. It is this calcium influx that is thought to initiate LTP induction (Lynch et al., 1983; Malenka et al., 1988). Calcium-sensitive signalling mechanisms, such as the calcium/calmodulin-dependent kinase II (CaMKII) or the cyclic adenosine monophosphate (cAMP)-dependent pathways, are then activated. These molecules, in turn, initiate LTP expression mechanisms, either locally, where they phosphorylate receptors and alter the intrinsic properties of their ligand-gated ion channels, or by signalling to the cell nucleus via transcription factors to alter gene expression (Goelet et al., 1986; Alberini et al., 1995). Expression mechanisms may be both pre- and post-synaptic (Kauer et al., 1988; Malgaroli and Tsien, 1992), with much current work focusing on changes in the intrinsic conductance of glutamate receptor channels (Derkach et al., 1999; Lee et al., 2003) or in the number inserted into the synaptic membrane (Takahashi et al., 2003). Structural changes to the shape or even number of synapses may also enhance the efficacy of potentiated pathways (Engert and Bonhoeffer, 1999). Many of these mechanisms are still controversial and none is completely characterized.
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Another important point is that the molecular mechanisms of LTP induction and maintenance vary somewhat from synapse to synapse. For instance, within the hippocampus, at the mossy fibre–CA3 pyramidal cell synapse, the NMDA receptor is not required for LTP induction (Harris and Cotman, 1986
) and the site of LTP expression is primarily pre-synaptic (Weisskopf and Nicoll, 1995). In contrast, LTP induction is mediated by the NMDA receptor at both medial perforant path–dentate gyrus granule cell (Morris et al., 1986; Errington et al. 1987) and Schaffer collateral–CA1 pyramidal cell synapses (Collingridge et al., 1983). Moreover, there is a major post-synaptic component to LTP expression at both the latter connections (McNaughton, 1982; Manabe et al., 1992; reviewed in Nicoll and Malenka, 1995). Even these two sets of synapses are dissociable in terms of signalling mechanisms, however, as CaMKII signalling is required at the latter but not the former (Zhang et al., 2005; Cooke et al., 2004). There is therefore no generalized picture for the molecular mechanisms supporting LTP induction and expression at glutamatergic synapses. It seems that the important properties of LTP, longevity, input specificity and associativity, can be implemented by a variety of receptors and signalling systems.
LTD is the converse process to LTP and results in a long-lasting decrease in synaptic efficacy. The standard protocol for inducing LTD uses long trains of low-frequency (1 Hz) stimulation (Dudek and Bear, 1992), or mismatching of pre- and post-synaptic action potentials (Markram et al., 1997). It seems likely that there are two mechanistically distinct forms of LTD: depotentiation, which refers to the reversal of LTP, and ‘de novo’ LTD, which refers to depression from an unpotentiated baseline. Some forms of LTD are dependent upon the NMDA receptor and are triggered by low concentrations of post-synaptic calcium (Nishiyama et al., 2000). Calcium-responsive phosphatases such as calcineurin and protein phosphatase 1 (PP1) are implicated as effector molecules in the mechanisms of LTD. These phosphatases dephosphorylate kinase targets such as glutamate receptors (Morishita et al., 2005) and the kinases themselves (Blitzer et al., 1998). LTD may serve as a homeostatic mechanism to ensure that CNS synapses are not saturated by learning. Alternatively, LTD may mediate learning in itself, forgetting or behavioural extinction.
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LTP and memory in rodents |
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A large body of evidence has now been gathered demonstrating that LTP and memory are supported by similar molecular mechanisms. Blockade of the NMDA receptor with antagonists such as 2-amino-5-phosphonopentanoic acid (AP5) impairs learning by rodents in a variety of hippocampus-dependent memory tasks. For example, when AP5 is infused into the hippocampus, rats are impaired in their ability to form a spatial map of the position of a hidden platform in the Morris water-maze, and the drug also prevents the induction of hippocampal LTP in these animals (Morris et al., 1986
; Abraham and Mason, 1988). Once a memory of the platform position has been acquired, AP5 infusions have no significant effect on the ability of the animal to locate the platform, just as the drug has no effect on a potentiated response once LTP has been induced. It seems, therefore, that the NMDA receptor is required for the induction of LTP, but plays little role either in baseline transmission or in the expression of the potentiated response (Morris et al., 1986).
Compelling evidence for the role of the NMDA receptor has come from a mutant mouse in which expression of the NR1 subunit of the NMDA receptor has been suppressed. This subunit is essential to the formation of a functional receptor, so the ‘knockout’ of this single gene in effect completely prevents the formation of functional NMDA receptors. Since NMDA receptors have vital roles early in the development of an organism, in addition to mediating synaptic plasticity in the adult, the NR1 knockout does not survive into post-natal life. In order to understand the role of the NMDA receptor in hippocampal LTP in the adult, a ‘conditional’ knockout mouse has been generated, in which knockout of the NR1 gene is restricted to the CA1 subfield of the hippocampus (Tsien et al., 1996). These mice survive well into adulthood, but fail to exhibit LTP at synapses in the CA1 subfield and also have specific spatial learning and memory deficits characteristic of hippocampal dysfunction. There is, therefore, strong correlative evidence to suggest a role for NMDA-receptor-dependent LTP in hippocampus-dependent learning and memory.
There are also common molecular features to the intracellular signalling mechanisms that mediate LTP and memory (see Fig. 2). Two of the major calcium-responsive signalling pathways that have been identified, CaMKII-dependent signalling and cAMP-dependent signalling, have been shown to participate in both LTP and learning and memory. The various isoforms of CaMKII are enzymes that respond to calcium when it is bound as a complex with the calcium-binding protein calmodulin. The activated enzyme then has the ability to sustain its own activity through autophosphorylation (Miller and Kennedy, 1986). Given this property, and its synaptic localization, it has long been mooted that CaMKII could in this way act as a local, self-perpetuating memory molecule (Lisman and Goldring, 1988; Lisman et al., 2002). The targeted mutation of a single amino acid, threonine 286, in the alphaCaMKII protein, prevents the autophosphorylation that allows the kinase to act autonomously in the absence of calcium. Mice carrying this mutation have a profound deficit in hippocampus-dependent learning and memory and also completely fail to exhibit LTP induction in the hippocampal CA1 subfield under standard stimulation protocols (Giese et al., 1998; Cooke et al., 2004). This alphaCaMKIIT286A point mutant mouse demonstrates the importance of alphaCaMKII in hippocampus-dependent memory and some forms of NMDA-receptor-dependent LTP, and fits well with the model in which autonomously active kinase can sustain LTP for some period of time after calcium concentration has subsided back to a basal level.
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The cAMP-dependent cascade is thought to mediate synapse to nucleus signalling and seems to initiate protein synthesis-dependent changes that take effect around an hour after LTP induction and that sustain both LTP and long-term memory in the long-term (Abel et al., 1997
; Nguyen and Kandel, 1997). The calcium-sensitivity of this pathway relies upon calcium/calmodulin-initiated conversion of adenosine triphosphate (ATP) into cAMP by adenylyl cyclase. Elevation of cAMP activates the cAMP-dependent protein kinase (PKA). Application of forskolin, which increases adenylyl cyclase activity and, thereby, the concentration of cAMP, induces LTP in hippocampal slices without the requirement for an electrical tetanus, an effect that can be blocked with PKA inhibitors (Frey et al., 1993). Over-expression of a regulatory PKA subunit has the effect of greatly reducing the activity of this enzyme, preventing both long-lasting LTP and long-term memory, although, in the short term, neither is affected (Abel et al., 1997). Over-expression of adenylyl cyclase in a transgenic mouse, which increases available cAMP levels, has the reverse effect and enhances LTP and learning (Wang et al., 2004).
The next step in this signalling pathway is the mitogen-activated kinase (MAPK). Activation of this enzyme by PKA is increased after hippocampus-dependent learning in mice and application of a MAPK inhibitor blocks the maintenance of LTP (Rosenblum et al., 2000; Rosenblum et al., 2002; Waltereit and Weller, 2003; Sweatt, 2004) and long-term memory formation (Atkins et al., 1998; Blum et al., 1999; Bozon et al., 2003). This cascade leads, indirectly, to the phosphorylation and thereby activation of cAMP-responsive element binding protein (CREB) in the nucleus. CREB is a transcription factor that governs the expression of a variety of LTP/memory effector proteins. The importance of CREB was initially demonstrated in invertebrates (Dash et al., 1990; Yin et al., 1994, 1995). Since then, both mutant mice with a targeted disruption of CREB and transgenic mice expressing a repressor of CREB have been shown to have deficits in sustainable LTP and hippocampus-dependent long-term memory (Bourtchuladze et al., 1994; Bozon et al., 2003) and mice expressing an inhibitor of an endogenous CREB repressor have both enhanced LTP and long-term memory storage (Chen et al., 2003). This series of results suggests an important role for the cAMP-dependent signalling cascade in long-lasting LTP and memory in mammals.
Overall, the literature now suggests a strong correlation between the effects of molecular and pharmacological manipulation on hippocampal LTP, and hippocampus-dependent learning and memory. Examples of mutant mice that have normal LTP but deficient memory have been presented in the literature (Migaud et al., 1998; Fragkouli et al., 2005). However, these findings do little to damage the hypothesis that synaptic plasticity is a key process in learning and memory because there are many other factors that may affect learning and memory. Attention, sensory acuity and anxiety, for example, are clearly also important variables. Very few neuroscientists would make the claim that LTP is sufficient for learning or memory. However, taking a simplistic overview, the hypothesis that synaptic plasticity is necessary for learning and memory could be disproven by a single example of a mutant mouse in which LTP is abolished, but learning and memory are normal. At first glance, just such a mouse appeared on the scene with the publication of a paper describing a mutant lacking the GluR1 subunit of the -amino-3-hydroxy-5-methylisoxazole-propionate (AMPA) receptor (Zamanillo et al., 1999). This knockout mouse performed as well as wild-type littermates in standard tests of hippocampus-dependent learning and memory, but LTP could not be induced in vitro in the CA1 subfield of the hippocampus using standard stimulation protocols. This, of course, is not the same as saying that LTP cannot under any circumstances be induced in vivo. Indeed, since the initial publication, it has been found that LTP can be induced at Schaffer collateral–CA1 synapses using a different and perhaps more physiologically realistic stimulation protocol (Hoffman et al., 2002). Moreover, deficits have now been reported in hippocampus-dependent learning and memory tasks in the GluR1 knockout mice (Reisel et al., 2005). All of these findings go to show that we will never entirely confirm or disprove a hypothesis that attempts to link LTP to behaviour by taking an essentially correlative approach (reviewed in Martin and Morris, 2002). Instead, by some as yet undetermined means we must test whether synaptic plasticity has an essential causal role in learning and memory. The most we can say at the moment is that synaptic plasticity, as modelled by LTP, is currently the favoured candidate mechanism for information storage within the CNS.
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Memory mechanisms in humans |
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Associative memory is commonly separated into two major divisions: declarative memory, which encompasses the recognition or recall of facts and episodes, and procedural memory, which refers to the retention of skills (Tulving, 1983
). Declarative memory can be further divided into episodic memory, which relates to autobiographical information, and semantic memory, which pertains specifically to non-autobiographical facts and propositions. To a great extent these divisions reflect the underlying neurobiology because different neural substrates mediate declarative and procedural memory. The medial temporal lobe has been a site of major interest in understanding declarative memory processes and, in particular, episodic memory, since the publication of a case study of the patient H.M. (Scoville and Milner, 1957). H.M. was a 23-year-old man who was subjected to a bilateral medial temporal lobectomy in an attempt to control severe epilepsy. As a consequence he was unable to form new episodic memories, although further studies showed that he had no deficit in procedural learning and memory. Studies of declarative memory have subsequently focused on the medial temporal lobe, and, in particular, the hippocampus. Functional magnetic resonance imaging (fMRI) studies suggest that the medial temporal lobes are involved in spatial (Rosenbaum et al., 2004), semantic (Bartha et al., 2003) and recognition memory (Kirwan and Stark, 2004), all of which fall under the banner of declarative memory, and studies of patients with medial temporal lobe damage have revealed a causal role for these structures in the same types of task (Teng and Squire, 1999; Manns et al., 2003a, b). The recognition of familiar and novel words is also believed to depend upon neural circuitry in the left medial temporal lobe (Bohbot et al., 1998).
Evidence for the involvement of molecular memory mechanisms similar to those identified in rodents comes from the finding that learning a variant of the Rey verbal memory task is prevented by systemic application of the NMDA receptor blocker ketamine in human subjects (Grunwald et al., 1999). Invasive recordings suggest that this may be due to an effect on neural plasticity. Measurements with implanted tungsten electrodes of event-related potentials (ERPs) in epileptic patients undergoing pre-surgery investigation reveal typical word-cued electrical responses, known as AMTL-N400s, in the medial temporal lobe, peaking at 
400 ms after presentation of the word (Elger et al., 1997). These responses normally undergo characteristic changes during learning in the Rey verbal test variant (Heit et al., 1990; Nobre and McCarthy, 1995). Interestingly, these changes in ERP are not sustained if words are presented and available to working memory but prevented from entering long-term memory by distracting patients during the key post-training consolidation period (Fernàndez et al., 1999). Memory-related changes in ERPs can be observed in medial temporal lobe structures, such as the hippocampus and rhinal cortex, but not in Wernicke's area, another region of cortex that plays an essential role in word processing and in which words elicit similar ERPs (Fernàndez et al., 1999). These findings are consistent with medial temporal lobe structures, but not Wernicke's area, being sites of storage for word-related memories. Significantly, the application of NMDA receptor antagonist ketamine prevents learning-related alteration of AMTL-N400s (Grunwald et al., 1999), strengthening the correlation between changes in medial temporal lobe ERPs and learning, and demonstrating that both are NMDA-receptor-dependent.
Further evidence for the involvement of molecular signalling pathways in human memory that are similar to those identified in rodents comes from recent attempts by the pharmaceutical industry to develop drugs to enhance memory. Many of this group of chemically unrelated substances, collectively known as nootropics, have been shown to enhance LTP in rodents. The most promising candidate nootropics for clinical use include modulators of AMPA receptors, NMDA receptors and the cAMP-dependent signalling cascade. AMPA receptor modulators, including the much-publicized AMPAkines, enhance LTP induction by prolonging the depolarization produced by synaptically released glutamate and thus facilitating activation of the NMDA receptor (Arai et al., 2004). One of these substances, CX-516, seems to have beneficial effects on learning and memory in humans and has reached phase II clinical trials for the treatment of Alzheimer's disease, dementia and schizophrenia (Goff et al., 2001). An alternative enhancement strategy directly targets the NMDA receptor. Memantine acts to enhance memory, in a somewhat contradictory fashion, by weakly antagonizing the NMDA receptor (Parsons et al., 1993). This seems to have beneficial effects on cognition in Alzheimer's disease (Lipton, 2005). NMDA-antagonism by memantine is thought to exert a nootropic effect in the long term as a result of protection from glutamate-induced excitotoxicity. It is important to note that memantine does not enhance memory through a direct modulation of LTP itself, although it does rescue deficits in LTP induced by excitotoxicity (Frankiewicz and Parsons, 1999). Another major strand of commercial and clinical investigation into nootropics has focused on the cAMP-dependent signalling pathway. Rolipram, an inhibitor of phosphodiesterases that increases the availability of cAMP and thereby increases the activity of PKA, enhances LTP and memory in rodents (Barad et al., 1998), most notably recovering some memory deficits in a mouse model of Alzheimer's disease (Gong et al., 2004). Rolipram has been considered a potential candidate for clinical use.
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LTP in humans |
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Investigations into LTP in humans are obviously limited. A rare opportunity for experiments comparable with those conducted in animal models has been provided by excision of hippocampal tissue from individuals undergoing surgery as a treatment for temporal lobe epilepsy (see Fig. 3). Careful treatment of this tissue after removal from the brain has enabled investigators to test some of the molecular features of LTP in the temporal cortex (Chen et al., 1996
) and, more recently, at human perforant path–granule cell synapses in the dentate gyrus (Beck et al., 2000). Substantial LTP can be induced in acute slices prepared from excised hippocampal tissue by brief tetanic stimulation of perforant path fibres. Potentiation of synaptic responses can be sustained for at least 2 h. Application of APS during the tetanus prevents the induction of LTP, demonstrating a requirement for the NMDA receptor, and sustained potentiation of synaptic responses results from bath application of forskolin, suggesting the involvement of the cAMP-dependent signalling pathway in LTP in humans.
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Patients contributing tissue to these studies fall into two groups: those with an epileptic focus in the hippocampus and those with a focus elsewhere in the temporal lobe. LTP can be readily induced in hippocampal tissue taken from patients with extra-hippocampal epileptic foci. The degree of LTP induced by tetanic stimulation in tissue taken from patients with hippocampal epileptic foci, however, is far more modest, and potentiation cannot be induced using forskolin. A possible reason for these observations is that synapses in epileptic tissue have become potentiated through epileptic activity, and are near saturation. A separate study found that expression of CaMKII is elevated in dentate granule cells of patients with hippocampal epileptic foci, perhaps reflecting a compensatory alteration of CaMKII signalling (Lie et al., 1998
). Finally, patients with hippocampal foci perform worse on the Rey verbal memory task than individuals with neo-cortical temporal lobe epileptic foci (Helmstaedter et al., 1997). This series of results from human subjects comprises a set of correlations between synaptic LTP, declarative memory, the NMDA receptor and intracellular signalling mechanisms that have previously been identified in animal models. |
Non-invasive stimulation in awake humans |
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Technical advances have presented the possibility of delivering tetanic stimulation to awake human subjects. This can be achieved using repetitive transcranial magnetic stimulation (rTMS), in which the cerebral cortex of an awake human subject can be stimulated non-invasively with a remote hand-held apparatus. Interventional paired associative stimulation (IPAS), which pairs TMS with electrical stimulation of peripheral nerves that provide input to the same cortical region, can be used in a similar manner. The risks of inducing seizure or long-lasting pathologies have had to be carefully evaluated before proceeding with experiments using remote stimulation with the high frequencies necessary for inducing LTP (Wassermann et al., 1996
). Experiments using these technologies have not focused on the medial temporal lobe for two major reasons. First, the hippocampus and surrounding structures lie deeper than 2 cm below the surface of the skull in humans, the current limiting distance for application of TMS (Bohning et al., 1997), and second, there is no well-defined behavioural output to use as a positive control of successful remote stimulation. For these reasons many TMS studies have been conducted in the motor cortex where remote stimulation can be used to elicit limb movements (Pridmore et al., 1998), typically in the hand. This positive control allows investigators to establish a motor threshold, which varies greatly from individual to individual, and set experimental stimulation intensity accordingly. In addition, monitoring motor output allows for the observation of long-term behavioural consequences of higher-frequency remote stimulation. |
Transcranial magnetic stimulation |
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TMS stimulation of motor cortex in humans using frequencies of 1–20 Hz produces effects on motor-evoked potentials that vary from individual to individual. Generally, 1 Hz stimulation reduces neural activity and anything over 5 Hz increases activity and motor output. In both cases the effects of such stimulation appear to be transient, lasting around half an hour at most (Hallett, 2000
). Interestingly, application of this low-frequency TMS to area M1 in the motor cortex can be used to block consolidation of motor skill acquisition in normal human subjects without interfering with motor performance itself (Muellbacher et al., 2002). Although changes in evoked potentials persist after the higher-frequency (5 Hz) trains of stimuli, the effect is not consistent and never lasts long enough to be comparable with LTP (Maeda et al., 2000). Higher-frequency tetani (50 Hz) have now been delivered and shown to be safe in normal individuals, provided the intensity of the stimulation is reduced to below motor threshold, although even this mode of stimulation does not produce changes that persist for longer than hundreds of milliseconds (Huang and Rothwell, 2004).
LTP is often induced in animals using repeated trains of high-frequency stimulation spaced at a frequency that mimics a spontaneous 5-7 Hz neural rhythm, the theta wave. Tetani of this sort via TMS can induce long-lasting changes in motor cortical output (Huang et al., 2005). Again the frequency of stimulation never exceeds 50 Hz in this sort of experiment [animal investigators may use frequencies as high as 400 Hz (Davis et al., 1997)], and the stimulation intensity must be set well below motor threshold during the tetanus. Nevertheless, the amplitude of motor-evoked potentials in the hand as a result of super-threshold baseline stimulation can increase by 50% for at least 20 min after application of several theta burst-like tetani spaced 10 s apart. This finding strongly suggests that remote stimulation can be used to induce a long-lasting change in motor cortical output. It has yet to be demonstrated, however, that the site of such change is the synapse.
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Interventional paired associative stimulation |
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As described above, an alternative means of inducing LTP that does not require the application of a high-frequency tetanus, is to pair pre- and post-synaptic action potentials (Wigstrom et al., 1987; Markram et al., 1997
; Bi and Poo, 1998). Pairing of this sort can potentially be modelled in humans by combining low-frequency TMS to the cortex whilst simultaneously stimulating a peripheral nerve, an approach known as IPAS (see Fig. 4). For example, peripheral stimulation of the right median nerve can be followed by TMS directed at the hand representation area in contralateral primary motor cortex (M1), at a latency determined by the time-lag in evoking an M1 cortical potential via activation of somatosensory cortex (Stefan et al., 2000). Motor-evoked potentials can again be used as an index of the resultant increase in motor cortical output, here in the abductor pollicis brevis muscle in the thumb. One benefit of using this approach compared with high-frequency TMS is that any risk of seizure is greatly reduced. Another is that it is more physiologically realistic and enables the testing of one of the key requirements for LTP—coincident pre- and post-synaptic activity. While coincident pre- and post-synaptic stimulation in the cortex, using peripheral stimulation preceding TMS stimulation, results in an increase in cortical excitability lasting for at least an hour (Stefan et al., 2000), mis-timing of peripheral and TMS stimulation, by shortening the interval between the two, results in a depression of cortical excitability lasting for an hour and a half (Wolters et al., 2003). Both of these effects can be blocked by the NMDA receptor antagonist dextromethorphan. This finding is consistent with the involvement of LTP/LTD-like processes. Moreover, the plasticity is limited to only those cells receiving stimulation in the cortex due to both peripheral stimulation and direct TMS, as demonstrated by the fact that there is no potentiation of motor-evoked responses in muscles controlled by neighbouring regions of motor cortex, such as the biceps brachii, which receive TMS stimulation but not peripherally induced stimulation. This experiment establishes that the potentiating effect is restricted to cells receiving paired input. Recent experiments reveal that motor learning prior to IPAS stimulation can prevent induction of the LTP-like plasticity in motor cortex for a period of 6 h (Stefan et al., 2005). Again, this finding suggests that the early motor learning may have saturated plasticity, thereby occluding further change. At the same time, the induction of LTD-like plasticity during this same period is facilitated (Ziemann et al., 2004), consistent with the idea that motor learning increases output from M1 through an LTP-like process, thereby allowing a greater scope for a reduction in the motor output, through depotentiation.
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Auditory and photic stimulation |
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An alternative to TMS has recently been used to induce long-lasting changes in neuronal excitability in human subjects, this time in the auditory (Clapp et al., 2005
) and visual cortices (Teyler et al., 2005). ERPs can be recorded in either area using scalp electrodes to monitor responses to auditory or visual stimuli. In these experiments, long-lasting enhancement of the amplitude of a component of either auditory-evoked or visual-evoked responses is achieved using a 13 Hz auditory tetanus, comprising a sequence of tone pips, or a photic tetanus generated on a computer screen, which comprises a series of chequerboard stimuli delivered at a frequency of 9 Hz. Either of these tetani is sufficient to increase the amplitude of a component of ERP in the respective area of cortex for at least 50 min afterwards. Moreover, in the latter case, delivery of lower-frequency visual stimuli (1 Hz) reduces the amplitude back to baseline levels, suggesting a depotentiation-like process.
The authors of these studies argue that the selective alteration of a single component of the ERP, which consists of electrical fields generated by a large number of neurons, constitutes a form of synaptic plasticity. This interpretation cannot be validated without more refined analysis, which, with the limits of current technology, is not yet possible. Nonetheless, it is a fascinating finding that a sensory tetanus alone can be used to induce long-lasting effects on neuronal responses in cerebral cortex. The finding complements animal studies in which LTP is induced at synapses made by fibres from projection neurons in the lateral geniculate nucleus on layer IV cells in the visual cortex pathway of rats (Heynen and Bear, 2001). Here LTP is induced by tetanic electrical stimulation, but subsequent to the tetanus, responses in primary visual cortex evoked by visual stimuli, such as light flashes and patterned gratings, are enhanced. The authors demonstrate that the potentiation is NMDA-receptor-dependent, using the NMDA receptor antagonist CPP [(±)-3-(2-carboxypiperazin-4-yl)-propyl-L-phosphonic acid] and indicate that the site of plasticity is synaptic, as revealed using current source density analysis (CSD; for an explanation of CSD methods, see Mitzdorf, 1985).
Regardless of the means of stimulation—whether TMS, IPAS, or photic or acoustic tetani—the end result is a long-lasting increase in cortical responsiveness. As yet, however, investigators have not been able to establish the exact nature of the underlying neural plasticity. Possibilities include changes in synaptic efficacy or in the threshold for action potential generation in the excitatory output cells of the cortex, or changes of similar sorts in intrinsic inhibitory networks. It is possible to observe alteration of activity in the neocortex using remote recording technology with electroencephalogram scalp electrodes (Clapp et al., 2005; Halder et al., 2005; Teyler et al., 2005), but non-invasive remote recording has poor spatial resolution and does not currently allow for discrimination between synaptic events and action potentials. EPSP-spike (E–S) potentiation, another form of neural plasticity in which the probability of an action potential being generated by a given synaptic input is increased, may well play a significant role in learning and memory (Giese et al., 2001). However, E–S potentiation is unlikely to provide the same capacity for information storage as potentiation of chemical transmission at individual synapses, because changes in the mechanism of action potential generation should, in theory, have an equivalent effect on many inputs to the same cell. Curiously, there is some evidence for a degree of input specificity in E–S plasticity (Douadal et al., 2002). However, it is likely that the specificity is limited to small populations of synapses rather than individual synapses.
At this point it is important to stress that the only direct evidence for synaptic plasticity in the human CNS comes from the experiments described earlier on excised human tissue (Chen et al., 1996; Beck et al., 2000). All those studies that we have described so far using remote means to induce changes in neuronal excitability and functional output of the human CNS in awake subjects are consistent with the induction of LTP or LTD at synapses. However, in order to demonstrate in these cases that change occurs in the efficacy of synaptic transmission rather than in the excitability of the cell, or in the balance of excitation and inhibition in the network in which the cell is embedded, it will be necessary to conduct experiments in which synaptic responses are monitored to activation of two clearly defined, and separately stimulated, input pathways. If change is synaptic it should be possible to potentiate or depress responses to one pathway without interfering with the other (see Fig. 3). Current approaches to non-invasive recording and stimulation have not yet allowed such observations to be made.
|
Neural plasticity and therapy |
|---|
Regardless of whether it is synaptic efficacy that is altered for long periods, or some other long-lasting form of neural plasticity, the net effect of the stimulation protocols described above is an increase in output from the neocortex. Repetitive stimulation of the brain can exert long-lasting functional effects, as demonstrated by the increased muscle activity in the hand in response to TMS directed at primary motor cortex. Treatment could potentially be provided for neurological disorders that arise from a reduction in the output of particular regions of the brain, as in Parkinson's disease and depression, using remote stimulation to induce long-lasting increases in excitatory drive. Currently available therapies using electrical stimulation rely upon invasive surgery. A non-invasive method of achieving the same end would obviously be preferable.
|
Depression |
|---|
Electroconvulsive therapy (ECT) has long been used to treat depression in cases in which other treatments fail (Potter and Rudorfer, 1993
). This is an extreme measure that, although effective in some cases, can also result in memory loss and other cognitive deficits (Frasca et al., 2003). A major benefit of using rTMS as a therapeutic treatment over ECT is that anaesthesia is not required. A number of studies have shown significant anti-depressant effects of rTMS (between 1 and 20 Hz), delivered to the prefrontal cortex, in patients with medication-resistant depression, as assessed using objective scales (George et al., 2000; Fitzgerald et al., 2003; Nahas et al., 2004), although effects are variable. It has been suggested that the use of theta burst rTMS, as recently demonstrated by Huang et al. (2005), may produce more consistent results because the effects appear to be longer-lasting than low-frequency stimulation (Paulus, 2005). A major concern with this method is the possibility that mania may result from increased activity in the same prefrontal areas targeted with rTMS (Kaptsan et al., 2003). Nonetheless, treatment of depression with rTMS is a promising avenue of clinical research. |
Parkinson's disease |
|---|
The use of rTMS to treat Parkinson's disease may be of less obvious therapeutic value. It is well known that the primary site of degeneration in this disease is a deep-lying midbrain structure—the substantia nigra. This is not accessible to remote stimulation with TMS. However, it is possible that some of the secondary effects of reduced nigral output, such as disrupted motor cortical activity, may be open to manipulation with non-invasive stimulation over the scalp. Basal ganglia dysfunction resulting in reduced nigral output results in characteristic synchronized activity in the motor cortex that is believed to contribute to akinesia and limb rigidity (Goldberg et al., 2002
). It has been shown that high-frequency stimulation targeted at the M1 area of the motor cortex can induce recovery from Parkinson's-like motor deficits in baboons treated with the toxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP). MPTP selectively kills dopaminergic neurons in the substantia nigra, thereby affecting basal ganglia function and initiating the parkinsonian symptoms of akinesia, bradykinesia, tremor and rigidity. Curiously, given the fact that motor cortical activity is not reduced by MPTP-induced pathology, but is instead simply highly synchronized, delivery of high-frequency (130 Hz) stimulation of the motor cortex, which presumably boosts motor cortical activity if it induces LTP, results in a significant long-lasting functional recovery from symptoms of akinesia and bradykinesia in MPTP-treated baboons (Drouot et al., 2004). The rationale behind this approach is somewhat counter-intuitive but the technique seems to produce results. Recently developed invasive therapies are beginning to yield some success in the treatment of Parkinson's disease (Houeto et al., 2002; Krack et al., 2003). In order to mimic the output of the substantia nigra, electrodes are implanted into the sub-thalamic nucleus or internal segment of the globus pallidus, basal ganglia components that lie downstream of the substantia nigra. However, this is a very difficult surgical procedure and involves significant risk. Non-invasive therapy may be preferable, even if it requires multiple treatments, so further research into the effects and side-effects of non-invasive motor cortex stimulation in Parkinson's models may yet lead to significant amelioration of symptoms. |
Epilepsy |
|---|
A final potential application for TMS is in the treatment of intractable epilepsy, a probable contributory factor to which is increased efficacy of glutamatergic synaptic transmission or reduced inhibition at a neuronal population level. It is possible that neuronal hyperexcitability in epilepsy could be reduced by induction of LTD. LTD may either de-potentiate over-potentiated synapses or compensate for other causes of hyperexcitability. Application of low-frequency rTMS (0.3 Hz) to epileptic foci in severely epileptic patients has been shown to have beneficial effects (Tergau et al., 1999
), reducing the number of seizures for a period of a month after stimulation. Further development of this approach seems warranted, given the demonstrations that it can be used to induce an LTD-like phenomenon in the neocortex (Chen et al., 1997; Ziemann et al., 2004). |
Hyperalgesia |
|---|
LTD induction may also have therapeutic value in the treatment of chronic neuropathic pain. Hyperalgesia of this sort can be modelled in rodents by injecting formalin subcutaneously into a paw. Alterations of central circuitry within the spinal cord occur that, in turn, mediate a long-lasting hypersensitivity to cutaneous stimulation around the conditioning site (Woolf et al., 1983
). This model has enabled the identification of cell types and signalling pathways involved in long-lasting central sensitization (reviewed in Han, 2003), and has also suggested the involvement of synaptic LTP in the induction of hyperalgesia (reviewed in Ji et al., 2003). Synapses between primary afferent peptidergic nociceptive fibres, which release substance P as a neurotransmitter, and projection neurons from lamina I of the dorsal horn of the spinal cord expressing the neurokinin 1 (NK1) receptor, which binds substance P, can display LTP in response to high-frequency stimulation. Neighbouring cells that receive nociceptive input but do not express the NK1 receptor do not exhibit LTP. The NK1 receptor is required for LTP at these synapses, as is the NMDA receptor and a rise in free intracellular Ca2+ (Liu and Sandkühler, 1997; Ikeda et al., 2003). Similar LTP occurs in rats using noxious stimulation itself, or following nerve injury (Sandkühler and Liu, 1998). If chronic neuropathic pain is mediated by LTP at a limited population of synapses then a logical approach to treatment would be to attempt to induce LTD at these same synapses. A recent study has used transcutaneous electrical nerve stimulation (TENS) to deliver high- and low-frequency tetani in human subjects in order to induce long-term hyper- and hypoalgesia, respectively, in response to mechanical stimulation of surrounding skin (Klein et al., 2004). Here ratings of pain levels by the subjects serve as an index of the degree of sensitization or analgesia. Although pain was reported to increase acutely during both high- and low-frequency stimulation, reported pain levels were persistently increased after high-frequency stimulation and decreased after low-frequency stimulation. LTD induced by low-frequency TENS, therefore, presents a potential avenue for therapy in neuropathic pain. |
Conclusion |
|---|
Thirty years of research into LTP has yielded a huge amount of data on the properties of longevity, input specificity and associativity, on the molecular mechanisms that support both short-lasting and persistent LTP, and on the correlation between LTP and learning and memory (reviewed in Bliss et al., 2003
, a themed issue of the Philosophical Transactions of the Royal Society of London). We are not yet in a position to conclude definitively that LTP provides a mechanism for the neural basis of learning and memory but it is certainly a compelling physiological model of these processes. Animal studies during the past three decades have covered a wide range of preparations, from dissociated cell cultures to awake, freely moving animals, but only recently has progress been made in the study of LTP in humans. Synaptic LTP can be induced in hippocampal tissue excised from human patients, and this plasticity, unsurprisingly, shares molecular mechanisms with animal models. Moreover, deficits in LTP are correlated with deficits in hippocampus-dependent memory in humans. Progress in remote stimulation technology is now making it possible to consider treatments based on the induction of long-lasting changes in cortical output using stimulation protocols similar to those that have been used to induce synaptic plasticity in animals. These LTP- and LTD-like effects may be of therapeutic value, and offer a potentially more targeted treatment for depression than ECT. Similar treatments may also be beneficial for other neurological disorders such as Parkinson's disease and epilepsy. |
References |
|---|
Abel T, Nguyen PV, Barad M, Deuel TA, Kandel ER, Bourtchouladze R. (1997) Genetic demonstration of a role for PKA in the late phase of LTP and in hippocampus-based long-term memory. Cell 88:615–26.[CrossRef][Web of Science][Medline]
Abraham WC and Mason SE. (1988) Effects of the NMDA receptor/channel antagonists CPP and MK801 on hippocampal field potentials and long-term potentiation in anesthetized rats. Brain Res 462:40–6.[CrossRef][Web of Science][Medline]
Abraham WC, Logan B, Greenwood JM, Dragunow M. (2002) Induction and experience-dependent consolidation of stable long-term potentiation lasting months in the hippocampus. J Neurosci 22:9626–34.
Alberini CM, Ghirardi M, Huang YY, Nguyen PV, Kandel ER. (1995) A molecular switch for the consolidation of long-term memory: cAMP-inducible gene expression. Ann NY Acad Sci 758:261–86.[Web of Science][Medline]
Andersen P, Sundberg SH, Sveen O, Swann JW, Wigström H. (1980) Possible mechanisms for long-lasting potentiation of synaptic transmission in hippocampal slices from guinea-pigs. J Physiol 302:463–82.
Arai AC, Xia YF, Suzuki E. (2004) Modulation of AMPA receptor kinetics differentially influences synaptic plasticity in the hippocampus. Neuroscience 123:1011–24.[CrossRef][Web of Science][Medline]
Atkins CM, Selcher JC, Petraitis JJ, Trzaskos JM, Sweatt JD. (1998) The MAPK cascade is required for mammalian associative learning. Nat Neurosci 1:602–9.[CrossRef][Web of Science][Medline]
Barad M, Bourtchouladze R, Winder DG, Golan H, Kandel E. (1998) Rolipram, a type IV-specific phosphodiesterase inhibitor, facilitates the establishment of long-lasting long-term potentiation and improves memory. Proc Natl Acad Sci USA 95:15020–5.
Barrionuevo G and Brown TH. (1983) Associative long-term potentiation in hippocampal slices. Proc Natl Acad Sci USA 80:7347–51.
Bartha L, Brenneis C, Schocke M, Trinka E, Koylu B, Trieb T, et al. (2003) Medial temporal lobe activation during semantic language processing: fMRI findings in healthy left- and right-handers. Brain Res Cogn Brain Res 17:339–46.[CrossRef][Medline]
Beck H, Goussakov IV, Lie A, Helmstaedter C, Elger CE. (2000) Synaptic plasticity in the human dentate gyrus. J Neurosci 20:7080–6.
Bi GQ and Poo MM. (1998) Synaptic modifications in cultured hippocampal neurons: dependence on spike timing, synaptic strength, and postsynaptic cell type. J Neurosci 18:10464–72.
Bliss TV and Lømo T. (1973) Long-lasting potentiation of synaptic transmission in the dentate area of the anaesthetized rabbit following stimulation of the perforant path. J Physiol 232:331–56.
Bliss TVP and Gardner-Medwin AR. (1973) Long-lasting potentiation of synaptic transmission in the dentate area of the unanaesthetized rabbit following stimulation of the perforant path. J Physiol 232:357–74.
In Bliss TVP, Collingridge GL, Morris RGM (Eds.). Long-term potentiation: enhancing neuroscience for 30 years. Philos Trans R Soc Lond B Biol Sci (2003) 358:603–842.
Blitzer RD, Connor JH, Brown GP, Wong T, Shenolikar S, Iyengar G, et al. (1998) Gating of CaMKII by cAMP-regulated protein phosphatase activity during LTP. Science 280:1940–2.
Blum S, Moore AN, Adams F, Dash PK. (1999) A mitogen-activated protein kinase cascade in the CA1/CA2 subfield of the dorsal hippocampus is essential for long-term spatial memory. J Neurosci 19:3535–44.
Bohbot VD, Kalina M, Stepankova K, Spackova N, Petrides M, Nadel L. (1998) Spatial memory deficits in patients with lesions to the right hippocampus and to the right parahippocampal cortex. Neuropsychologia 36:1217–38.[CrossRef][Web of Science][Medline]
Bohning DE, Pecheny AP, Epstein CM, Speer AM, Vincent DJ, Dannels W, et al. (1997) Mapping transcranial magnetic stimulation (TMS) fields in vivo with MRI. Neuroreport 8:2535–8.[Web of Science][Medline]
Bourtchuladze R, Frenguelli B, Blendy J, Cioffi D, Schutz G, Silva AJ. (1994) Deficient long-term memory in mice with a targeted mutation of the cAMP-responsive element-binding protein. Cell 79:59–68.[CrossRef][Web of Science][Medline]
Bozon B, Kelly A, Josselyn SA, Silva AJ, Davis S, Laroche S. (2003) MAPK, CREB and zif268 are all required for the consolidation of recognition memory. Philos Trans R Soc Lond B Biol Sci 358:805–14.
Chen WR, Lee S, Kato K, Spencer DD, Shepherd GM, Williamson A. (1996) Long-term modifications of synaptic efficacy in the human inferior and middle temporal cortex. Proc Natl Acad Sci USA 93:8011–5.
Chen R, Classen J, Gerloff C, Celnik P, Wassermann EM, Hallett M, et al. (1997) Depression of motor cortex excitability by low-frequency transcranial magnetic stimulation. Neurology 48:1398–403.
Chen A, Muzzio IA, Malleret G, Bartsch D, Verbitsky M, Pavlidis P, et al. (2003) Inducible enhancement of memory storage and synaptic plasticity in transgenic mice expressing an inhibitor of ATF4 (CREB-2) and C/EBP proteins. Neuron 39:655–69.[CrossRef][Web of Science][Medline]
Clapp WC, Kirk IJ, Hamm JP, Shepherd D, Teyler TJ. (2005) Induction of LTP in the human auditory cortex by sensory stimulation. Eur J Neurosci 22:1135–40.[CrossRef][Web of Science][Medline]
Collingridge GL, Kehl SJ, McLennan H. (1983) Excitatory amino acids in synaptic transmission in the Schaffer collateral-commissural pathway of the rat hippocampus. J Physiol 334:33–46.
Cooke SF, Errington ML, Plattner F, Bradshaw KD, Hirano A, Peters M, et al. (2004) Long-term potentiation at perforant path-granule cell synapses does not require phosphorylation of ?-CaMKII at threonine 286. Forum European Neurosci Abstr 4.AO15.13.
Davis S, Bliss TV, Dutrieux G, Laroche S, Errington ML. (1997) Induction and duration of long-term potentiation in the hippocampus of the freely moving mouse. J Neurosci Methods 75:75–80.[CrossRef][Web of Science][Medline]
Dash PK, Hochner B, Kandel ER. (1990) Injection of the cAMP-responsive element into the nucleus of Aplysia sensory neurons blocks long-term facilitation. Nature 345:718–21.[CrossRef][Medline]
Derkach V, Barria A, Soderling TR. (1999) Ca2+/calmodulin-kinase II enhances channel conductance of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate type glutamate receptors. Proc Natl Acad Sci USA 96:3269–74.
Daoudal G, Hanada Y, Debanne D. (2002) Bidirectional plasticity of excitatory postsynaptic potential (EPSP)-spike coupling in CA1 hippocampal pyramidal neurons. Proc Natl Acad Sci USA 99:14512–7.
Drouot X, Oshino S, Jarraya B, Besret L, Kishima H, Remy P, et al. (2004) Functional recovery in a primate model of Parkinson's disease following motor cortex stimulation. Neuron 44:769–78.[CrossRef][Web of Science][Medline]
Dudek SM and Bear MF. (1992) Homosynaptic long-term depression and effects of N-methyl-D-aspartate receptor blockade. Proc Natl Acad Sci USA 89:4363–7.
Elger CE, Grunwald T, Lehnertz K, Kutas M, Helmstaedter C, Brockhaus A, et al. (1997) Human temporal lobe potentials in verbal learning and memory processes. Neuropsychologia 35:657–67.[CrossRef][Web of Science][Medline]
Engert F and Bonhoeffer T. (1999) Dendritic spine changes associated with hippocampal long-term synaptic plasticity. Nature 399:66–70.[CrossRef][Medline]
Errington ML, Lynch MA, Bliss TV. (1987) Long-term potentiation in the dentate gyrus: induction and increased glutamate release are blocked by D(–)aminophosphonovalerate. Neuroscience 20:279–84.[CrossRef][Web of Science][Medline]
Fernàndez G, Effern A, Grunwald T, Pezer N, Lehnertz K, Dümpelmann M, et al. (1999) Real-time tracking of memory formation in the human rhinal cortex and hippocampus. Science 285:1582–5.
Fitzgerald PB, Brown TL, Marston NA, Daskalakis ZJ, De Castella A, Kulkarni J. (2003) Transcranial magnetic stimulation in the treatment of depression: a double-blind, placebo-controlled trial. Arch Gen Psychiatry 60:1002–8.
Fox K. (2002) Anatomical pathways and molecular mechanisms for plasticity in the barrel cortex. Neuroscience 111:799–814.[CrossRef][Web of Science][Medline]
Fragkouli A, Hearn C, Errington M, Cooke S, Grigoriou M, Bliss T, et al. (2005) Loss of forebrain cholinergic neurons and impairment in spatial learning and memory in LHX7-deficient mice. Eur J Neurosci 21:2923–38.[CrossRef][Web of Science][Medline]
Frankiewicz T and Parsons CG. (1999) Memantine restores long term potentiation impaired by tonic N-methyl-D-aspartate (NMDA) receptor activation following reduction of Mg2+ in hippocampal slices. Neuropharmacology 38:1253–9.[CrossRef][Web of Science][Medline]
Frasca TA, Iodice A, McCall WV. (2003) The relationship between changes in learning and memory after right unilateral electroconvulsive therapy. J ECT 19:148–50.[CrossRef][Web of Science][Medline]
Frey U, Huang YY, Kandel ER. (1993) Effects of cAMP simulate a late stage of LTP in hippocampal CA1 neurons. Science 260:1661–4.
George MS, Nahas Z, Molloy M, Speer AM, Oliver NC, Li XB, et al. (2000) A controlled trial of daily left prefrontal cortex TMS for treating depression. Biol Psychiatry 48:962–70.[CrossRef][Web of Science][Medline]
Giese KP, Fedorov NB, Filipkowski RK, Silva AJ. (1998) Autophosphorylation at Thr286 of the alpha calcium-calmodulin kinase II in LTP and learning. Science 279:870–3.
Giese KP, Peters M, Vernon J. (2001) Modulation of excitability as a learning and memory mechanism: a molecular genetic perspective. Physiol Behav 73:803–10.[CrossRef][Medline]
Goelet P, Castellucci VF, Schacher S, Kandel ER. (1986) The long and the short of long-term memory—a molecular framework. Nature 322:419–22.[CrossRef][Medline]
Goff DC, Leahy L, Berman I, Posever T, Herz L, Leon AC, et al. (2001) A placebo-controlled pilot study of the ampakine CX516 added to clozapine in schizophrenia. J Clin Psychopharmacol 21:484–7.[CrossRef][Web of Science][Medline]
Goldberg JA, Boraud T, Maraton S, Haber SN, Vaadia E, Bergman H. (2002) Enhanced synchrony among primary motor cortex neurons in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine primate model of Parkinson's disease. J Neurosci 22:4639–53.
Gong B, Vitolo OV, Trinchese F, Liu S, Shelanski M, Arancio O. (2004) Persistent improvement in synaptic and cognitive functions in an Alzheimer mouse model after rolipram treatment. J Clin Invest 114:1624–34.[CrossRef][Web of Science][Medline]
Grunwald T, Beck H, Lehnertz K, Blumcke I, Pezer N, Kurthen M, et al. (1999) Evidence relating human verbal memory to hippocampal N-methyl-D-aspartate receptors. Proc Natl Acad Sci USA 96:12085–9.
Gustafsson B, Wigstrom H, Abraham WC, Huang YY. (1987) Long-term potentiation in the hippocampus using depolarizing current pulses as the conditioning stimulus to single volley synaptic potentials. J Neurosci 7:774–80.[Abstract]
Halder P, Sterr A, Brem S, Bucher K, Kollias S, Brandeis D. (2005) Electrophysiological evidence for cortical plasticity with movement repetition. Eur J Neurosci 21:2271–7.[CrossRef][Web of Science][Medline]
Hallett M. (2000) Transcranial magnetic stimulation and the human brain. Nature 406:147–50.[CrossRef][Medline]
Han J. (2003) Acupuncture: neuropeptide release produced by electrical stimulation at different frequencies. Trends Neurosci 26:17–22.[CrossRef][Web of Science][Medline]
Harris EW and Cotman CW. (1986) Long-term potentiation of guinea pig mossy fibre responses is not blocked by N-methyl-D-aspartate antagonists. Neurosci Lett 70:132–7.[CrossRef][Web of Science][Medline]
Hebb DO. (1949) The organization of behavior (Wiley, New York).
Heit G, Smith ME, Halgren E. (1990) Neuronal activity in the human medial temporal lobe during recognition memory. Brain 113:1093–112.
Helmstaedter C, Grunwald T, Lehnertz K, Gleissner U, Elger CE. (1997) Differential involvement of left temporolateral and temporomesial structures in verbal declarative learning and memory: evidence from temporal lobe epilepsy. Brain Cogn 35:110–31.[CrossRef][Web of Science][Medline]
Heynen AJ and Bear MF. (2001) Long-term potentiation of thalamocortical transmission in the adult visual cortex in vivo. J Neurosci 21:9801–13.
Hoffman DA, Sprengel R, Sakmann B. (2002) Molecular dissection of hippocampal theta-burst pairing potentiation. Proc Natl Acad Sci USA 28:7740–5.
Houeto JL, Mesnage V, Mallet L, Pillon B, Gargiulo M, du Moncel ST, et al. (2002) Behavioural disorders, Parkinson's disease and subthalamic stimulation. J Neurol Neurosurg Psychiatry 72:701–7.
Huang YZ and Rothwell JC. (2004) The effect of short-duration bursts of high-frequency, low-intensity transcranial magnetic stimulation on the human motor cortex. Clin Neurophysiol 115:1069–75.[CrossRef][Web of Science][Medline]
Huang YZ, Edwards MJ, Rounis E, Bhatia KP, Rothwell JC. (2005) Theta burst stimulation of the human motor cortex. Neuron 45:201–6.[CrossRef][Web of Science][Medline]
Ikeda H, Heinke B, Ruscheweyh R, Sandkuhler J. (2003) Synaptic plasticity in spinal lamina I projection neurons that mediate hyperalgesia. Science 299:1237–40.
Ji RR, Kohno T, Moore KA, Woolf CJ. (2003) Central sensitization and LTP: do pain and memory share similar mechanisms? Trends Neurosci 26:696–705.[CrossRef][Web of Science][Medline]
Kaptsan A, Yaroslavsky Y, Applebaum J, Belmaker RH, Grisaru N. (2003) Right prefrontal TMS versus sham treatment of mania: a controlled study. Bipolar Disord 5:36–9.[Web of Science][Medline]
Kauer JA, Malenka RC, Nicoll RA. (1988) A persistent postsynaptic modification mediates long-term potentiation in the hippocampus. Neuron 1:911–7.[CrossRef][Web of Science][Medline]
Kirwan CB and Stark CE. (2004) Medial temporal lobe activation during encoding and retrieval of novel face-name pairs. Hippocampus 14:919–30.[CrossRef][Web of Science][Medline]
Klein T, Magerl W, Hopf HC, Sandkuhler J, Treede RD. (2004) Perceptual correlates of nociceptive long-term potentiation and long-term depression in humans. J Neurosci 24:964–71.
Krack P, Batir A, Van Blercom N, Chabardes S, Fraix V, Ardouin C, et al. (2003) Five-year follow-up of bilateral stimulation of the subthalamic nucleus in advanced Parkinson's disease. N Engl J Med 349:1925–34.
Lee HK, Takamiya K, Han JS, Man H, Kim CH, Rumbaugh G, et al. (2003) Phosphorylation of the AMPA receptor GluR1 subunit is required for synaptic plasticity and retention of spatial memory. Cell 89:684–90.
Levy WB and Steward O. (1979) Synapses as associative memory elements in the hippocampal formation. Brain Res 175:233–45.[CrossRef][Web of Science][Medline]
Lie AA, Blumcke I, Beck H, Schramm J, Wiestler OD, Elger CE. (1998) Altered patterns of Ca2+/calmodulin-dependent protein kinase II and calcineurin immunoreactivity in the hippocampus of patients with temporal lobe epilepsy. J Neuropathol Exp Neurol 57:1078–88.[Web of Science][Medline]
Lipton SA. (2005) The molecular basis of memantine action in Alzheimer's disease and other neurologic disorders: low-affinity, uncompetitive antagonism. Curr Alzheimer Res 2:155–65.[CrossRef][Medline]
Lisman JE and Goldring MA. (1988) Feasibility of long-term storage of graded information by the Ca2+/calmodulin-dependent protein kinase molecules of the postsynaptic density. Proc Natl Acad Sci USA 85:5320–4.
Lisman J, Schulman H, Cline H. (2002) The molecular basis of CaMKII function in synaptic and behavioural memory. Nature Rev Neurosci 3:175–90.[CrossRef][Web of Science][Medline]
Liu X and Sandkuhler J. (1997) Characterization of long-term potentiation of C-fiber-evoked potentials in spinal dorsal horn of adult rat: essential role of NK1, NK2 receptors. J Neurophysiol 78:1973–82.
Lynch G, Larson J, Kelso S, Barrionuevo G, Schottler F. (1983) Intracellular injections of EGTA block induction of hippocampal long-term potentiation. Nature 305:719–21.[CrossRef][Medline]
Maeda F, Keenan JP, Tormos JM, Topka H, Pascual-Leone A. (2000) Interindividual variability of the modulatory effects of repetitive transcranial magnetic stimulation on cortical excitability. Exp Brain Res 133:425–30.[CrossRef][Web of Science][Medline]
Malenka RC, Kauer JA, Zucker RS, Nicoll RA. (1988) Postsynaptic calcium is sufficient for potentiation of hippocampal synaptic transmission. Science 242:81–4.
Malgaroli A and Tsien RW. (1992) Glutamate-induced long-term potentiation of the frequency of miniature synaptic currents in cultured hippocampalneurons. Nature 357:134–9.[CrossRef][Medline]
Manabe T, Renner P, Nicoll RA. (1992) Postsynaptic contribution to long-term potentiation revealed by analysis of miniature synaptic currents. Nature 355:50–5.[CrossRef][Medline]
Manns JR, Hopkins RO, Reed JM, Kitchener EG, Squire LR. (2003a) Recognition memory and the human hippocampus. Neuron 37:171–80.[CrossRef][Web of Science][Medline]
Manns JR, Hopkins RO, Squire LR. (2003b) Semantic memory and the human hippocampus. Neuron 38:127–33.[CrossRef][Web of Science][Medline]
Markram H, Lubke J, Frotscher M, Sakmann B. (1997) Regulation of synaptic efficacy by coincidence of postsynaptic APs and EPSPs. Science 275:213–5.
Martin SJ and Morris RG. (2002) New life in an old idea: the synaptic plasticity and memory hypothesis revisited. Hippocampus 12:609–36.[CrossRef][Web of Science][Medline]
McNaughton BL. (1982) Long-term synaptic enhancement and short-term potentiation in rat fascia dentata act through different mechanisms. J Physiol 324:249–62.
McNaughton BL, Douglas RM, Goddard GV. (1978) Synaptic enhancement in fascia dentata: cooperativity among coactive afferents. Brain Res 157:277–93.[CrossRef][Web of Science][Medline]
Migaud M, Charlesworth P, Dempster M, Webster LC, Watabe AM, Makhinson M, et al. (1998) Enhanced long-term potentiation and impaired learning in mice with mutant postsynaptic density-95 protein. Nature 396:433–9.[CrossRef][Medline]
Miller SG and Kennedy MB. (1986) Regulation of brain type II Ca2+/calmodulin-dependent protein kinase by autophosphorylation: a Ca2+-triggered molecular switch. Cell 44:861–70.[CrossRef][Web of Science][Medline]
Mitzdorf U. (1985) Current source-density method and application in cat cerebral cortex: investigation of evoked potentials and EEG phenomena. Physiol Rev 65:37–100.
Morishita W, Marie H, Malenka RC. (2005) Distinct triggering and expression mechanisms underlie LTD of AMPA and NMDA synaptic responses. Nat Neurosci 8:1043–50.[CrossRef][Web of Science][Medline]
Morris RG, Anderson E, Lynch GS, Baudry M. (1986) Selective impairment of learning and blockade of long-term potentiation by an N-methyl-D-aspartate receptor antagonist, AP5. Nature 319:774–6.[CrossRef][Medline]
Muellbacher W, Ziemann U, Wissel J, Dang N, Kofler M, Facchini S, et al. (2002) Early consolidation in human primary motor cortex. Nature 415:640–4.[CrossRef][Medline]
Nahas Z, Li X, Kozel FA, Mirzki D, Memon M, Miller K, et al. (2004) Safety and benefits of distance-adjusted prefrontal transcranial magnetic stimulation in depressed patients 55–75 years of age: a pilot study. Depress Anxiety 19:249–56.[CrossRef][Web of Science][Medline]
Nguyen PV and Kandel ER. (1997) Brief theta-burst stimulation induces a transcription-dependent late phase of LTP requiring cAMP in area CA1 of the mouse hippocampus. Learn Mem 4:230–43.
Nicoll RA and Malenka RC. (1995) Contrasting properties of two forms of long-term potentiation in the hippocampus. Nature 377:115–8.[CrossRef][Medline]
Nishiyama M, Hong K, Mikoshiba K, Poo M-M, Kato K. (2000) Calcium stores regulate the polarity and input specificity of synaptic modification. Nature 408:584–8.[CrossRef][Medline]
Nobre AC and McCarthy G. (1995) Language-related field potentials in the anterior-medial temporal lobe: II. Effects of word type and semantic priming. J Neurosci 15:1090–8.[Abstract]
Nosten-Bertrand M, Errington ML, Murphy KP, Tokugawa Y, Barboni E, Kozlova E, et al. (1996) Normal spatial learning despite regional inhibition of LTP in mice lacking Thy-1. Nature 379:826–9.[CrossRef][Medline]
Nowak L, Bregestovski P, Ascher P, Herbet A, Prochiantz A. (1984) Magnesium gates glutamate-activated channels in mouse central neurones. Nature 307:462–5.[CrossRef][Medline]
Parsons CG, Gruner R, Rozental J, Millar J, Lodge D. (1993) Patch clamp studies on the kinetics and selectivity of N-methyl-D-aspartate receptor antagonism by memantine (1-amino-3,5–dimethyladamantan). Neuropharmacology 32:1337–50.[CrossRef][Web of Science][Medline]
Paulus W. (2005) Toward establishing a therapeutic window for rTMS by theta burst stimulation. Neuron 45:181–3.[CrossRef][Web of Science][Medline]
Potter WZ and Rudorfer MV. (1993) Electroconvulsive therapy—a modern medical procedure. N Engl J Med 328:882–3.
Pridmore S, Fernandes Filho JA, Nahas Z, Liberatos C, George MS. (1998) Motor threshold in transcranial magnetic stimulation: a comparison of a neurophysiological method and a visualization of movement method. J ECT 14:25–7.[Web of Science][Medline]
Reisel D, Bannerman DM, Deacon RM, Sprengel R, Seeburg PH, Rawlins JN. (2005) GluR-A-dependent synaptic plasticity is required for the temporal encoding of nonspatial information. Behav Neurosci 119:1298–306.[CrossRef][Web of Science][Medline]
Rosenblum K, Futter M, Jones M, Hulme EC, Bliss TV. (2000) ERKI/II regulation by the muscarinic acetylcholine receptors in neurons. J Neurosci 20:977–85.
Rosenblum K, Futter M, Voss K, Erent M, Skehel PA, French P, et al. (2002) The role of extracellular regulated kinases I/II in late-phase long-term potentiation. J Neurosci 22:5432–41.
Rosenbaum RS, Ziegler M, Winocur G, Grady CL, Moscovitch M. (2004) ‘I have often walked down this street before’: fMRI studies on the hippocampus and other structures during mental navigation of an old environment. Hippocampus 14:826–35.[CrossRef][Web of Science][Medline]
Sandkuhler J and Liu X. (1997) Induction of long-term potentiation at spinal synapses by noxious stimulation or nerve injury. Eur J Neurosci 10:2476–80.
Scoville WB and Milner B. (1957) Loss of recent memory after bilateral hippocampal lesions. J Neurol Neurosurg Psychiatry 20:11–21.[Medline]
Skrede KKR and Westgaard RH. (1971) The transverse hippocampal slice: a well-defined cortical structure maintained in vitro. Brain Res 35:589–93.[CrossRef][Web of Science][Medline]
Stefan K, Kunesch E, Cohen LG, Benecke R, Classen J. (2000) Induction of plasticity in the human motor cortex by paired associative stimulation. Brain 123:572–84.
Stefan K, Wycislo M, Gentner R, Schramm A, Naumann M, Reiners K, et al. (2006) Temporary occlusion of associative motor cortical plasticity by prior dynamic motor training. Cereb Cortex 16:376–85.
Sweatt JD. (2004) Mitogen-activated protein kinases in synaptic plasticity and memory. Current Opin Neurobiol 14:311–7.[CrossRef][Web of Science][Medline]
Takahashi T, Svoboda K, Malinow R. (2003) Experience strengthening transmission by driving AMPA receptors into synapses. Science 299:1585–8.
Teng E and Squire LR. (1999) Memory for places learned long ago is intact after hippocampal damage. Nature 400:675–7.[CrossRef][Medline]
Tergau F, Naumann U, Paulus W, Steinhoff BJ. (1999) Low-frequency repetitive transcranial magnetic stimulation improves intractable epilepsy. Lancet 353:2209.[CrossRef][Web of Science][Medline]
Teyler TJ, Hamm JP, Clapp WC, Johnson BW, Corballis MC, Kirk IJ. (2005) Long-term potentiation of human visual evoked responses. Eur J Neurosci 21:2045–50.[Web of Science][Medline]
Tsien JZ, Huerta PT, Tonegawa S. (1996) The essential role of hippocampal CA1 NMDA receptor-dependent synaptic plasticity in spatial memory [see comments]. Cell 87:1327–38.[CrossRef][Web of Science][Medline]
Tulving E. (1983) Elements of episodic memory. (Clarendon Press, Oxford).
Urban NN, Henze DA, Lewis DA, Barrionuevo G. (1996) Properties of LTP induction in the CA3 region of the primate hippocampus. Learn Mem 3:86–95.
Waltereit R and Weller M. (2003) Signaling from cAMP/PKA to MAPK and synaptic plasticity. Mol Neurobiol 27:99–106.[CrossRef][Web of Science][Medline]
Wang H, Ferguson GD, Pineda VV, Cundiff PE, Storm DR. (2004) Overexpression of type-1 adenylyl cyclase in mouse forebrain enhances recognition memory and LTP. Nat Neurosci 7:635–42.[CrossRef][Web of Science][Medline]
Wassermann EM, Cohen LG, Flitman SS, Chen R, Hallett M. (1996) Seizures in healthy people with repeated ‘safe’ trains of transcranial magnetic stimuli. Lancet 347:825–6.[Web of Science][Medline]
Weisskopf MG and Nicoll RA. (1995) Presynaptic changes during mossy fibre LTP revealed by NMDA receptor-mediated synaptic responses. Nature 376:256–9.[CrossRef][Medline]
Wigstrom H, Gustafsson B, Huang YY, Abraham WC. (1986) Hippocampal long-term potentiation is induced by pairing single afferent volleys with intracellularly injected depolarizing current pulses. Acta Physiol Scand 126:317–9.[Web of Science][Medline]
Wolters A, Sandbrink F, Schlottmann A, Kunesch E, Stefan K, Cohen LG, et al. (2003) A temporally asymmetric Hebbian rule governing plasticity in the human motor cortex. J Neurophysiol 89:2339–45.
Woolf CJ. (1983) Evidence for a central component of post-injury pain hypersensitivity. Nature 306:686–8.[CrossRef][Medline]
Yin JC, Wallach JS, Del Vecchio M, Wilder EL, Zhou H, Quinn WG, et al. (1994) Induction of a dominant negative CREB transgene specifically blocks long-term memory in Drosophila. Cell 79:49–58.[CrossRef][Web of Science][Medline]
Yin JC, Del Vecchio M, Zhou H, Tully T. (1995) CREB as a memory modulator: induced expression of a dCREB2 activator isoform enhances long-term memory in Drosophila. Cell 81:107–15.[CrossRef][Web of Science][Medline]
Zamanillo D, Sprengel R, Hvalby O, Jensen V, Burnashev N, Rozov A, et al. (1999) Importance of AMPA receptors for hippocampal synaptic plasticity but not for spatial learning. Science 284:1805–11.
Zhang L, Kirschstein T, Sommersberg B, Merkens M, Manahan-Vaughan D, Elgersma Y, et al. (2005) Hippocampal synaptic metaplasticity requires inhibitory autophosphorylation of Ca2+/calmodulin-dependent kinase II. J Neurosci 25:7697–707.
Ziemann U, Ilic TV, Pauli C, Meintzschel F, Ruge D. (2004) Learning modifies subsequent induction of long-term potentiation-like and long-term depression-like plasticity in human motor cortex. J Neurosci 24:1666–72.
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