Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow An erratum has been published
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (79)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Jacobsen, M.
Right arrow Articles by Hemmer, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Jacobsen, M.
Right arrow Articles by Hemmer, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Brain, Vol. 125, No. 3, 538-550, March 2002
© 2002 Guarantors of Brain

Oligoclonal expansion of memory CD8+ T cells in cerebrospinal fluid from multiple sclerosis patients

Marc Jacobsen1, Sabine Cepok1, Elfriede Quak1, Michael Happel1, Rami Gaber1, Andreas Ziegler2, Sabine Schock1, Wolfgang H. Oertel1, Norbert Sommer1 and Bernhard Hemmer1

1 Clinical Neuroimmunology Group, Department of Neurology, Philipps University, Rudolf-Bultmann Strasse 8 and 2 Institute of Medical Biometry and Epidemiology, Philipps University, Bunsenstrasse 3, 35033 Marburg, Germany

Correspondence to: B. Hemmer, Clinical Neuroimmunology Group, Department of Neurology, Philipps University, Rudolf-Bultmann Strasse 8, 35033 Marburg, Germany E-mail: hemmer{at}mailer.uni-marburg.de

Multiple sclerosis is a chronic inflammatory demyelinating disease of the CNS. Although the aetiology of multiple sclerosis is still unknown, it is widely believed that T cells play a central role in its pathogenesis. To identify and characterize disease-relevant T cells, we analysed CD4+ and CD8+ T cells freshly isolated from the CSF and peripheral blood of 36 multiple sclerosis patients for their T-cell receptor variable ß (TCRBV) chain repertoire. In most patients, we found significant overexpression of individual TCRBV chains on CD8+ T cells from CSF compared with peripheral blood. In contrast, only a few multiple sclerosis patients showed differences between the two compartments in TCRBV expression on CD4+ T cells. The overexpression of specific TCRBV chains on CD8+ T cells was found to be stable over several months in selected patients and involved mainly T cells with a memory phenotype. In two patients studied, individual TCRBV chain overexpression was found to be caused by the expansion of T cell populations with identical or highly similar rearranged T-cell receptor ß- and {alpha}-chain sequences, which were not found among peripheral blood CD8+ T cells. Our findings demonstrate selective enrichment of memory CD8+ T cells in the CSF of multiple sclerosis patients, suggesting a role for these CD8+ T cells in the pathogenesis of multiple sclerosis. Our study provides a basis for future trials to identify disease-associated antigens and disease pathogenesis in multiple sclerosis.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 BrainHome page
S. Jilek, M. Schluep, P. Meylan, F. Vingerhoets, L. Guignard, A. Monney, J. Kleeberg, G. Le Goff, G. Pantaleo, and R. A. Du Pasquier
Strong EBV-specific CD8+ T-cell response in patients with early multiple sclerosis
Brain, July 1, 2008; 131(7): 1712 - 1721.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. T. Mars, J. Bauer, D. A. Gross, F. Bucciarelli, H. Firat, D. Hudrisier, F. Lemonnier, K. Kosmatopoulos, and R. S. Liblau
CD8 T Cell Responses to Myelin Oligodendrocyte Glycoprotein-Derived Peptides in Humanized HLA-A*0201-Transgenic Mice
J. Immunol., October 15, 2007; 179(8): 5090 - 5098.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
I. Galea, M. Bernardes-Silva, P. A. Forse, N. van Rooijen, R. S. Liblau, and V. H. Perry
An antigen-specific pathway for CD8 T cells across the blood-brain barrier
J. Exp. Med., September 3, 2007; 204(9): 2023 - 2030.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Jacobsen, A. K. Detjen, H. Mueller, A. Gutschmidt, S. Leitner, U. Wahn, K. Magdorf, and S. H. E. Kaufmann
Clonal Expansion of CD8+ Effector T Cells in Childhood Tuberculosis
J. Immunol., July 15, 2007; 179(2): 1331 - 1339.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
P. Somma, G. Ristori, L. Battistini, S. Cannoni, G. Borsellino, A. Diamantini, M. Salvetti, R. Sorrentino, and M. T. Fiorillo
Characterization of CD8+ T cell repertoire in identical twins discordant and concordant for multiple sclerosis
J. Leukoc. Biol., March 1, 2007; 81(3): 696 - 710.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
J. A. Black, S. Liu, B. C. Hains, C. Y. Saab, and S. G. Waxman
Long-term protection of central axons with phenytoin in monophasic and chronic-relapsing EAE
Brain, December 1, 2006; 129(12): 3196 - 3208.
[Abstract] [Full Text] [PDF]

Home page
 Arch NeurolHome page
O. Stuve, C. M. Marra, A. Bar-Or, M. Niino, P. D. Cravens, S. Cepok, E. M. Frohman, J. T. Phillips, G. Arendt, K. R. Jerome, et al.
Altered CD4+/CD8+ T-Cell Ratios in Cerebrospinal Fluid of Natalizumab-Treated Patients With Multiple Sclerosis.
Arch Neurol, October 1, 2006; 63(10): 1383 - 1387.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Brisebois, S. P. Zehntner, J. Estrada, T. Owens, and S. Fournier
A Pathogenic Role for CD8+ T Cells in a Spontaneous Model of Demyelinating Disease
J. Immunol., August 15, 2006; 177(4): 2403 - 2411.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
M. A. Friese, X. Montalban, N. Willcox, J. I. Bell, R. Martin, and L. Fugger
The value of animal models for drug development in multiple sclerosis
Brain, August 1, 2006; 129(8): 1940 - 1952.
[Abstract] [Full Text] [PDF]

Home page
 Mult SclerHome page
E Fainardi, R Rizzo, L Melchiorri, M Castellazzi, E Paolino, M R Tola, E Granieri, and O R Baricordi
Intrathecal synthesis of soluble HLA-G and HLA-I molecules are reciprocally associated to clinical and MRI activity in patients with multiple sclerosis
Multiple Sclerosis, February 1, 2006; 12(1): 2 - 12.
[Abstract] [PDF]

Home page
 NeuroscientistHome page
C. Beeton and K. G. Chandy
Potassium Channels, Memory T Cells, and Multiple Sclerosis
Neuroscientist, December 1, 2005; 11(6): 550 - 562.
[Abstract] [PDF]

Home page
 BrainHome page
M. A. Friese and L. Fugger
Autoreactive CD8+ T cells in multiple sclerosis: a new target for therapy?
Brain, August 1, 2005; 128(8): 1747 - 1763.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
H. Pellkofer, A. S. Schubart, R. Hoftberger, N. Schutze, M. Pagany, M. Schuller, H. Lassmann, R. Hohlfeld, R. Voltz, and C. Linington
Modelling paraneoplastic CNS disease: T-cells specific for the onconeuronal antigen PNMA1 mediate autoimmune encephalomyelitis in the rat
Brain, August 1, 2004; 127(8): 1822 - 1830.
[Abstract] [Full Text] [PDF]

Home page
 BrainHome page
D.-A. Laplaud, C. Ruiz, S. Wiertlewski, S. Brouard, L. Berthelot, M. Guillet, B. Melchior, N. Degauque, G. Edan, P. Brachet, et al.
Blood T-cell receptor {beta} chain transcriptome in multiple sclerosis. Characterization of the T cells with altered CDR3 length distribution
Brain, May 1, 2004; 127(5): 981 - 995.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. Skulina, S. Schmidt, K. Dornmair, H. Babbe, A. Roers, K. Rajewsky, H. Wekerle, R. Hohlfeld, and N. Goebels
Multiple sclerosis: Brain-infiltrating CD8+ T cells persist as clonal expansions in the cerebrospinal fluid and blood
PNAS, February 24, 2004; 101(8): 2428 - 2433.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M. Schroeter, G. Stoll, R. Weissert, H.-P. Hartung, H. Lassmann, and S. Jander
CD8+ Phagocyte Recruitment in Rat Experimental Autoimmune Encephalomyelitis: Association with Inflammatory Tissue Destruction
Am. J. Pathol., October 1, 2003; 163(4): 1517 - 1524.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.