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Brain Advance Access originally published online on March 14, 2006
Brain 2006 129(5):1319-1329; doi:10.1093/brain/awl057
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© The Author (2006). Published by Oxford University Press on behalf of the Guarantors of Brain. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Remyelination of dorsal column axons by endogenous Schwann cells restores the normal pattern of Nav1.6 and Kv1.2 at nodes of Ranvier

Joel A. Black1,2, Stephen G. Waxman1,2 and Kenneth J. Smith3

1 Department of Neurology and Center for Neuroscience and Regeneration Research, Yale School of Medicine, New Haven, 2 Rehabilitation Research Center, VA Connecticut Healthcare System, West Haven, CT, USA and 3 Department of Clinical Neuroscience, King's College London, Guy's Campus, London, UK

Correspondence to: Joel A. Black, PhD Neuroscience Research (127A), VA Connecticut Healthcare System, 950 Campbell Avenue, West Haven, CT 06518, USA E-mail joel.black{at}yale.edu

Demyelination of CNS axons occurs in a number of pathological conditions, including multiple sclerosis and contusion-type spinal cord injury. The demyelination can be repaired by remyelination in both humans and rodents, and even within the CNS remyelination can be achieved by endogenous and/or exogenous Schwann cells, the myelinating cells of the PNS. Remyelinated axons can often conduct impulses securely, but the organization of ion channels at long-term remyelinated nodes is not known. In the present study, the expression of voltage-gated sodium (Nav) and potassium (Kv) channels along central axons remyelinated by endogenous Schwann cells has been studied in lesions induced more than 1 year previously by the intraspinal injection of ethidium bromide (EB). The expression of the channels at long-term nodes formed by Schwann cell remyelination has been compared with that present in nascent nodes formed in the adult at 18 and 23 days post-EB injection. Immunohistochemical studies revealed that long-term nodes formed by Schwann cell remyelination exhibit a clustering of Nav1.6 sodium channels within the nodal membrane, with the Shaker-type potassium channel Kv1.2 segregated within the juxtaparanodal region, similar to the arrangement at normal mature CNS nodes. Nav1.2 was not detected at nodes formed by Schwann cells at any stage of their development. Moreover, Nav1.6, but not Nav1.2, was clustered at nascent nodes formed by remyelinating Schwann cells 18 and 23 days following EB injection. These observations show that endogenous Schwann cells can establish and maintain nodes of Ranvier on central axons for over one year, and that the nodes exhibit an apparently normal distribution of sodium and potassium channels, with Nav1.6 the predominant subtype of sodium channel present at such nodes at all stages of their development.

Key Words: demyelination; remyelination; Schwann cell; sodium channel; spinal cord

Abbreviations: Caspr = contactin-associated protein; EB = ethidium bromide; MOG = myelin oligodendrocyte glycoprotein; Nav = voltage-gated sodium; Kv = voltage-gated potassium

Received September 20, 2005. Revised December 14, 2005. Accepted February 10, 2006.


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