Brain Advance Access originally published online on September 21, 2007
Brain 2007 130(11):2789-2799; doi:10.1093/brain/awm214
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Multiple sclerosis: T-cell receptor expression in distinct brain regions
1Institute for Clinical Neuroimmunology, Ludwig Maximilians University, D-81377 Munich, 2Department of Neuroimmunology, Max-Planck-Institute for Neurobiology, D-82152 Martinsried, Germany and 3Center for Brain Research, Medical University of Vienna, A-1090 Vienna, Austria
Correspondence to: Klaus Dornmair or Reinhard Hohlfeld, Institute for Clinical Neuroimmunology, Maximilians University, D-81377 Munich, Germany E-mail: dornmair{at}neuro.mpg.de; reinhard.hohlfeld{at}med.uni-muenchen.de
Multiple sclerosis (MS) is an inflammatory demyelinating disease where T cells attack the brain and the spinal cord. It is known that often particular T-cell clones are expanded in the target tissue, but it is still unknown, whether identical T-cell clones are present at distinct anatomical sites, or whether the T-cell spectrum is locally diverse. Therefore we compared the T-cell receptor (TCR) repertoire in distinct lesions and normal-appearing white matter (NAWM) from post-mortem brains of four MS patients. We analysed 19 lesions (inactive demyelinated, 15; slowly expanding chronic, 3; active lesions, 1) and 5 NAWM regions. The TCR ß-chain repertoire was investigated by CDR3 spectratyping. For each anatomical site 325 semi-nested PCR reactions were performed. About 800 Vß-NDN-Jß combinations were sequenced. Each of the four patients had distinct T-cell clones that were present in more than two anatomically distinct regions. These clones were not restricted to lesions, but were also present in NAWM. Some clones were present in all investigated lesions, and additionally, in NAWM sites. A single T-cell clone was detected in nine different sites in one patient. None of the clones was shared among different patients. Thus, pervasive T-cell clones exist in distinct regions of MS brain, and these clones are ‘private’ (unique) to individual patients. Analysis of the hypervariable NDN region revealed ‘silent’ nucleotide exchanges, i.e. nucleotide exchanges that code for identical amino acids. Such silent nucleotide exchanges suggest that the corresponding T-cell clones were recruited and stimulated by particular antigens. To attribute some of the pervasive clones to particular T-cell subsets, we isolated individual CD8+ T cells from cryosections by laser microdissection and characterized their TCR by single-cell PCR. These experiments revealed that at least some of the pervasive T-cell clones belonged to the CD8+ compartment, supporting the pathogenic relevance of this T-cell subset.
Key Words: multiple sclerosis; T-cell receptor; autoimmune T cells; neuroimmunology; cytotoxic T-cell response
Abbreviations: CDR, complementarity determining region; NAWM, normal-appearing white matter; TCR, T-cell receptor
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Received June 19, 2007. Revised August 13, 2007. Accepted August 14, 2007.
*These authors contributed equally to this work.
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