Brain Advance Access published online on August 22, 2003
Brain, doi:10.1093/brain/awg277
© 2003 by Guarantors of Brain
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Article
1 Department of Molecular Cardiology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, OH, USA
* Corresponding author. E-mail: perezd{at}ccf.org.
Received 21 April 2003
; revised 30 May 2003
; accepted 24 June 2003
The
Keywords: adrenergic; microarray; NMDA; GABA; transgenic
Gene expression profile of neurodegeneration induced by
1B-adrenergic receptor overactivity: NMDA/GABAA dysregulation and apoptosis
2 Department of Neurology, The Cleveland Clinic Foundation, Cleveland, OH, USA
1-adrenergic receptors (1ARs) play an important role in mediating sympathetic neurotransmission in peripheral organ systems; however, central 1ARs are not well characterized. Additionally, due to the lack of sufficiently subtype-selective drugs or high avidity antibodies, the contribution of each 1AR subtype to various central functions is currently unclear. Transcription regulation through 1AR subtypes in the CNS is also unknown. Of interest, transgenic mice that systemically overexpress the 1BAR show central symptoms that include age-progressive impaired mobility, neurodegeneration and susceptibility to epileptic seizure. To investigate the molecular basis of this phenotype, oligonucleotide microarray studies of whole brains of various ages were carried out to compare gene expression profiles between transgenic and normal brains. The results indicated changes in expression of apoptotic, calcium regulatory, neurodegenerative and genes involved in neurotransmission. Defects in regulation of intracellular calcium are known to play a role in cell death; thus, these genes may provide clues as to the molecular basis of 1BAR-induced neurodegeneration. Epilepsy is a disorder that can be caused by an imbalance between excitatory (e.g. glutamate) and inhibitory (e.g. GABA) signals. Microarray analysis of transgenic brains showed increased N-methyl-D-aspartate (NMDA) receptors and decreased GABAA, which were confirmed with immunohistochemistry, western blot and radioligand binding studies. The 1BAR also co-localized with the glutamatergic distribution, suggesting a glutamate imbalance as a molecular rationale for the epileptic seizures.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. K. Gupta, R. S. Papay, C. W. D. Jurgens, R. J. Gaivin, T. Shi, V. A. Doze, and D. M. Perez {alpha}1-Adrenergic Receptors Regulate Neurogenesis and Gliogenesis Mol. Pharmacol., August 1, 2009; 76(2): 314 - 326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E Zeisig, B-O Ljung, H Alfredson, and P Danielson Immunohistochemical evidence of local production of catecholamines in cells of the muscle origins at the lateral and medial humeral epicondyles: of importance for the development of tennis and golfer's elbow? Br. J. Sports Med., April 1, 2009; 43(4): 269 - 275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. L. Hillman, V. A. Doze, and J. E. Porter {alpha}1A-Adrenergic Receptors Are Functionally Expressed by a Subpopulation of Cornu Ammonis 1 Interneurons in Rat Hippocampus J. Pharmacol. Exp. Ther., June 1, 2007; 321(3): 1062 - 1068. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Debrus, L. Rahbani, M. Marttila, B. Delorme, P. Paradis, and M. Nemer The Zinc Finger-Only Protein Zfp260 Is a Novel Cardiac Regulator and a Nuclear Effector of {alpha}1-Adrenergic Signaling Mol. Cell. Biol., October 1, 2005; 25(19): 8669 - 8682. [Abstract] [Full Text] [PDF]
|
|