Skip Navigation


Brain Advance Access first published online on May 4, 2005
This version published online on May 25, 2005
Brain, doi:10.1093/brain/awh517
This Article
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
128/8/1897    most recent
awh517v2
awh517v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Wang, X.
Right arrow Articles by Gu, X.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wang, X.
Right arrow Articles by Gu, X.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author (2005). Published by Oxford University Press on behalf of the Guarantors of Brain. All rights reserved. For Permissions, please email: journals.permissions@oupjournals.org
Received October 28, 2004
Revised March 28, 2005
Accepted March 29, 2005

Article

Dog sciatic nerve regeneration across a 30-mm defect bridged by a chitosan/PGA artificial nerve graft

Xiaodong Wang 1, Wen Hu 1, Yong Cao 1, Jian Yao 1, Jian Wu 1, and Xiaosong Gu 1*

1 Key Laboratory of Neuroregeneration, Nantong University, Nantong City, Jiangsu, People's Republic of China

* To whom correspondence should be addressed.
Xiaosong Gu, E-mail: neurongu{at}public.nt.js.cn


  Abstract

We have developed a dual-component artificial nerve graft comprising an outer microporous conduit of chitosan and internal oriented filaments of polyglycolic acid (PGA). The novel graft was used for bridging sciatic nerve across a 30-mm defect in six Beagle dogs, which were used as a chitosan/PGA graft group. The other Beagle dogs were divided into an autograft group (n = 6) as the positive control and a non-grafted group (n = 5) as the negative control. All animals of three groups were monitored for changes in their appearance and locomotion activities after surgery. Their posture and gait were recorded regularly with the aid of photographs and videotapes for each dog. Six months post-operatively, a combination of electrophysiological examination, FluoroGold retrograde tracing, histological assessment including light microscopy and transmission electron microscopy, immunohistochemistry as well as morphometric analyses to both regenerated nerves and target muscles was utilized to investigate the nerve repair effects of our artificial nerve graft. The results demonstrated that, in the chitosan/PGA graft group, the dog sciatic nerve trunk had been reconstructed with restoration of nerve continuity and functional recovery, and its target skeletal muscle had been re-innervated, improving locomotion activities of the operated limb. This study proves the feasibility of the chitosan/PGA artificial nerve graft for peripheral nerve regeneration by bridging a longer defect in a large animal model.

Keywords: artificial nerve graft; chitosan; polyglycolic acid; long sciatic nerve defects; dogs.
Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Biomater ApplHome page
M. Patel, P. J. Vandevord, H. W. Matthew, S. De Silva, Bin Wu, and P. H. Wooley
Collagen--Chitosan Nerve Guides for Peripheral Nerve Repair: A Histomorphometric Study
J Biomater Appl, September 1, 2008; 23(2): 101 - 121.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.