Brain Advance Access published online on July 1, 2009
Brain, doi:10.1093/brain/awp163
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TASK1 modulates inflammation and neurodegeneration in autoimmune inflammation of the central nervous system
1 University of Wuerzburg, Department of Neurology, Josef-Schneider-Str. 11, 97080 Wuerzburg, Germany 2 Westfaelische Wilhelms-University Muenster, Institute of Physiology, Robert-Koch Str. 27a, 48149 Muenster, Germany 3 University of Virginia, Department of Pharmacology, Charlottesville, Virginia 22908, USA 4 University of Heidelberg, Department of Neuroradiology, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany
Correspondence to:
Sven G. Meuth, MD, PhD, University of Wuerzburg, Department of Neurology, Josef-Schneider-Str. 11, 97080 Wuerzburg, Germany E-mail: meuth_s{at}klinik.uni-wuerzburg.de Correspondence may also be addressed to: Heinz Wiendl, E-mail: heinz.wiendl{at}klinik.uni-wuerzburg.de
We provide evidence that TWIK-related acid-sensitive potassium channel 1 (TASK1), a member of the family of two-pore domain potassium channels relevant for setting the resting membrane potential and balancing neuronal excitability that is expressed on T cells and neurons, is a key modulator of T cell immunity and neurodegeneration in autoimmune central nervous system inflammation. After induction of experimental autoimmune encephalomyelitis, an experimental model mimicking multiple sclerosis, TASK1–/– mice showed a significantly reduced clinical severity and markedly reduced axonal degeneration compared with wild-type controls. T cells from TASK1–/– mice displayed impaired T cell proliferation and cytokine production, while the immune repertoire is otherwise normal. In addition to these effects on systemic T cell responses, TASK1 exhibits an independent neuroprotective effect which was demonstrated using both a model of acutely prepared brain slices cocultured with activated T cells as well as in vitro cultivation experiments with isolated optic nerves. Anandamide, an endogenous cannabinoid and inhibitor of TASK channels, reduced outward currents and inhibited effector functions of T cells (IFN-
production and proliferation); an effect completely abrogated in TASK1–/– mice. Accordingly, preventive blockade of TASK1 significantly ameliorated experimental autoimmune encephalomyelitis after immunization. Therapeutic application of anandamide significantly reduced disease severity and was capable of lowering progressive loss of brain parenchymal volume as assessed by magnetic resonance imaging. These data support the identification and characterization of TASK1 as potential molecular target for the therapy of inflammatory and degenerative central nervous system disorders.
Key Words: multiple sclerosis; EAE; TASK1; K2P; potassium channels
Abbreviations: AEA, arachidonylethanolamide; APC, antigen-presenting cells; AVD, apoptotic volume decrease; EAE, experimental autoimmune encephalomyelitis; CST, corticospinal tract; PBS, phosphate-buffered saline; PFA, paraformaldehyde
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Received January 15, 2009. Revised May 10, 2009. Accepted May 12, 2009.
*These authors contributed equally to this work.