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Default network connectivity reflects the level of consciousness in non-communicative brain-damaged patients

Audrey Vanhaudenhuyse, Quentin Noirhomme, Luaba J.-F. Tshibanda, Marie-Aurelie Bruno, Pierre Boveroux, Caroline Schnakers, Andrea Soddu, Vincent Perlbarg, Didier Ledoux, Jean-François Brichant, Gustave Moonen, Pierre Maquet, Michael D. Greicius, Steven Laureys, Melanie Boly
DOI: http://dx.doi.org/10.1093/brain/awp313 161-171 First published online: 23 December 2009


The ‘default network’ is defined as a set of areas, encompassing posterior-cingulate/precuneus, anterior cingulate/mesiofrontal cortex and temporo-parietal junctions, that show more activity at rest than during attention-demanding tasks. Recent studies have shown that it is possible to reliably identify this network in the absence of any task, by resting state functional magnetic resonance imaging connectivity analyses in healthy volunteers. However, the functional significance of these spontaneous brain activity fluctuations remains unclear. The aim of this study was to test if the integrity of this resting-state connectivity pattern in the default network would differ in different pathological alterations of consciousness. Fourteen non-communicative brain-damaged patients and 14 healthy controls participated in the study. Connectivity was investigated using probabilistic independent component analysis, and an automated template-matching component selection approach. Connectivity in all default network areas was found to be negatively correlated with the degree of clinical consciousness impairment, ranging from healthy controls and locked-in syndrome to minimally conscious, vegetative then coma patients. Furthermore, precuneus connectivity was found to be significantly stronger in minimally conscious patients as compared with unconscious patients. Locked-in syndrome patient’s default network connectivity was not significantly different from controls. Our results show that default network connectivity is decreased in severely brain-damaged patients, in proportion to their degree of consciousness impairment. Future prospective studies in a larger patient population are needed in order to evaluate the prognostic value of the presented methodology.

  • Default mode
  • fMRI
  • coma
  • vegetative state
  • minimally conscious state


In recent years, advances in emergency medicine and reanimation have considerably increased the number of patients surviving prolonged cardiac arrest or severe motor vehicle accidents (Laureys and Boly, 2008). An important proportion of these surviving patients are left with severe brain damage, leading to the presence of disorders of consciousness. Among disorders of consciousness, coma is defined by ‘unarousable unresponsiveness’; and ‘vegetative state’ by preserved behavioural sleep–wake cycles and reflexive but not purposeful behaviours (Laureys and Boly, 2007). Minimally conscious patients, though unable to communicate, show inconsistent non-reflexive behaviours, interpreted as signs of awareness of self or environment (Giacino et al., 2002). The locked-in syndrome describes patients who are awake and conscious but have no means of producing speech, limb or facial movements (American Congress of Rehabilitation Medicine, 1995). A particular problem in patients with disorders of consciousness is that the clinical diagnosis is very challenging at the bedside, and several studies have reported high rates of misdiagnosis, reaching up to 40% (Majerus et al., 2005; Schnakers et al., 2009). It is now increasingly recognized that diagnosing these distinct conditions correctly is critical, both for ethical reasons and in order to improve the clinical management of these patients. Indeed, several studies have shown that brain activation in response to auditory or painful stimuli is very limited when in a vegetative state, while this activation is virtually normal in patients who are minimally conscious (Boly et al., 2004, 2008a), suggesting the possibility of residual external stimuli perception in the latter patient population. Furthermore, preliminary data show that patients in the minimally conscious state have a much better functional prognosis than patients in a vegetative state, independently of the aetiology (Giacino, 2005). These concerns raise the need for reliable paraclinical markers as a complement to the clinical assessment in differentiating patients in a vegetative state from patients in a minimally conscious state.

Over the last 8 years, increasing attention has been paid to the study of spontaneous brain activity and its significance for cognition and behaviour (Raichle, 2006). In particular, the concept of a ‘default mode network (DMN) of brain function’ was introduced by Raichle et al. (2001), after observing that a number of areas including the precuneus, bilateral temporo-parietal junctions and medial prefrontal cortex, were more active at rest than when the subjects were involved in an attention-demanding cognitive task. This network of areas, now commonly referred to as the ‘DMN’, has been replicably implicated in cognitive processes like ‘daydreaming’ or ‘mind-wandering’, stimulus-independent thoughts or self-related thoughts (Laureys et al., 2007; Mason et al., 2007; Buckner et al., 2008). Though the functional significance of the DMN remains a matter of debate, it has been suggested as a candidate for the network subserving basic functions related to consciousness (Boly et al., 2008b; Greicius et al., 2008). Studying this network in patients with disorders of consciousness is, at first glance, a very challenging undertaking, due to the highly subjective and complex cognitive functions reported to be supported by this network.

Several studies in healthy volunteers have shown the ability of resting state functional magnetic resonance imaging (fMRI) to identify structured patterns of functional connectivity among defined neuroanatomical systems reliably, including the DMN (Cavanna and Trimble, 2006; Damoiseaux et al., 2006; Cavanna, 2007; Shehzad et al., 2009). Of potentially major interest from the clinical point of view, is the fact that resting state fMRI connectivity studies allow the investigation of higher order cognitive networks like the DMN, without requiring the patients’ collaboration. This fact is particularly important in non-communicative patients such as those with disorders of consciousness. Resting state fMRI acquisitions are also very easy to perform compared with standard task-based fMRI paradigms, and could thus have a potentially broader and faster translation into clinical practice. However, to date, the functional significance of resting state connectivity patterns remain unclear. Some authors have even questioned the value and interpretability of spontaneous brain activity fluctuations as recorded by fMRI (Morcom and Fletcher, 2007).

The aim of this study was to investigate DMN resting state fMRI connectivity in a cohort of patients with disorders of consciousness including coma, vegetative state, minimally conscious state and locked-in syndrome. We hypothesized that DMN connectivity strength would be related to the level of consciousness of non-communicative brain-damaged patients, as assessed by standardized behavioural scales. Furthermore, we expected a particularly strong link between the level of consciousness and connectivity in the precuneus, reported to be a central node in the DMN (Fransson and Marrelec, 2008), and potentially the most connected area in the brain (Hagmann et al., 2008).



We compared 14 brain-injured patients (1 locked-in syndrome, 4 minimally conscious, 4 vegetative state and 5 coma patients, age range 25–77 years) to 14 age-matched healthy volunteers (age range 28–57 years). In patients, clinical examination was repeatedly performed using standardized scales [the Coma Recovery Scale-Revised (CRS-R) (Giacino et al., 2004); and the Glasgow Liege scale (Born, 1988)] on the day of scanning, and in the week before and the week after. Table 1 reports demographic and clinical characteristics of the patients. Patients were scanned in an unsedated condition. The study was approved by the Ethics Committee of the Medical School of the University of Liège. Informed consent to participate to the study was obtained from the subjects themselves in the case of healthy subjects, and from the legal surrogate of the patients.

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Table 1

Clinical, electrophysiological and structural imaging data of patients

Clinical Features
Sex (age, years)Male (25)Male (69)Female (57)Male (75)Male (26)Female (76)Female (76)Male (71)Female (46)
CauseTraumaCRAHaemorrhageEncephalitisTraumaCO intoxicationStrokeEncephalitisStroke
Time of fMRI (days after insult)1593623695 years16343816
Outcome at 12 monthsGOS 2GOS 1GOS 3GOS 1GOS 3GOS 3GOS 3GOS 1GOS 1
BreathingSpontaneousSpontaneous— with tubeSpontaneousSpontaneousSpontaneousSpontaneousSpontaneousSpontaneousSpontaneous
Paralysis/paresisTetraparesisTetraparesisTetraparesisTetraparesisTetraparesisTetraparesisTetrapraesisTetraparesisTetraplegia Preserved vertical occulomotricity
Auditory functionStartle reflexNoneStartle reflexStartle reflexReproducible movement to commandStartle reflexStartle reflexStartle reflexConsistent movement to command
Visual functionNoneNoneBlink to threatNoneVisual pursuitVisual fixationVisual pursuitVisual pursuitObject recognition
Motor functionFlexion to painNoneNoneFlexion to painFlexion to painNoneAutomatic motor responseFlexion to painFlexion to pain
Oromotor/Verbal functionOral reflexesOral reflexesOral reflexesNoneOral reflexesOral reflexesOral reflexesOral reflexesOral reflexes
ArousalWith stimulationWith stimulationWith stimulationWithout stimulationWithout stimulationWith stimulationWithout stimulationWithout stimulationAttention
Total score524411512916
Background activityBilateral very slow delta and intermittent thetaTheta with intermittent diffuse deltaDelta-theta irregularDiffuse delta activityLow voltage theta, muscular artefactsSymmetric theta–delta activityDelta–theta, predominantly on the right sideTheta activityTheta – with signs of reticular formation impairment
Increased intensity on T2Diffuse axonal injury (thalamus, mesencephalon, bilateral grey matter)Bilateral sylvian and frontal lesionsBilateral frontoparietal lesionsCentro- protuberantial and bilateral white matter lesionsDiffuse leucoencephalopathy and cerebral atrophy Subcortical lesions in basal ganglia and thalamusBilateral pallidal lesionsRight frontotemporal lesionCerebellar peduncles lesionsBulbo-medullar junction and cerebellar pedoncules lesions
Clinical Features
Sex (age, years)Female (49)Male (34)Male (40)Male (48)Female (77)
CauseMeningioma coma post-surgeryAnoxiaCRAHaemorrhageStroke
Time of fMRI (days after admission)1477513
Outcome at 12 monthsGOS 1GOS 4GOS 1GOS 1GOS 1
BreathingSpontaneousSpontaneousSpontaneous with tubeSpontaneousSpontaneous with tube
Diagnosis at time of fMRIComaComaComaComaComa
Auditory functionNoneNoneNoneNoneStartle reflex
Visual functionNoneBlink to threatNoneNoneNone
Motor functionAbnormal posturingFlexion to painAbnormal posturingNoneFlexion to pain
Oromotor/Verbal functionNoneNoneNoneNoneOral reflexes
Total score13104
Background activityTheta–delta bilateralIntermittent theta – delta Abundant spike-wakes and spikesGeneralized status epilepticusBilateral posterior thetaGeneralized status epilepticus
Increased intensity on T2Petroclival tumour with cavernous sinus and bulbomedullar junction invasionNoneDiffuse cortical, basal basal ganglia oedema and right capsulo-thalamic lesionsLeft temporo-occipital and cerebral peduncles, tegmentum and vermis lesionsLeft pulvinar and ascending reticular formation
  • GOS = Glasgow Outcome Scale; LIS = locked-in syndrome; MCS = minimally conscious state; VS = vegetative state.

Data acquisition and analysis

In all subjects, 10 min resting state fMRI were acquired on a 1.5 T magnetic resonance scanner (Siemens, Germany). Two hundred multislice T2*-weighted fMRI images were obtained with a gradient echo-planar sequence using axial slice orientation (36 slices; voxel size: 3.75 × 3.75 × 3.6 mm3; matrix size 64 × 64 × 36; repetition time = 3000 ms; echo time = 30 ms; flip angle = 90°; field of view = 240 mm). Head movements were minimized using customized cushions. A T1 magnetization prepared rapid gradient echo sequence was also acquired in the same session for coregistration with functional data. Monitoring of vital parameters (electrocardiogram, blood pressure, pulse oxymetry, end tidal carbon dioxide partial pressure and respiratory rate) was performed in patients by a senior anaesthesiologist throughout the experiment.

Data analysis was performed using Statistical Parametric Mapping-5 (www.fil.ion.ucl.ac.uk/spm) and probabilistic independent component analysis (Beckmann and Smith, 2004) as implemented in MELODIC, part of the Functional MRI of the Brain software library (FSL) (www.fmrib.ox.ac.uk/fsl). Independent component analysis is a statistical technique that separates a set of signals into independent (uncorrelated and non-Gaussian) spatio-temporal components (Beckmann and Smith, 2004). When applied to the T2* signal of fMRI, independent component analysis allows not only for the removal of artefacts (McKeown et al., 1998; Quigley et al., 2002), but also for the isolation of task-activated neural networks (McKeown et al., 1998; Calhoun et al., 2002), or of low-frequency neural networks during task-free or cognitively undemanding fMRI scans (Greicius et al., 2004; Beckmann et al., 2005; Seeley et al., 2007). In a first step, functional images were re-aligned, normalized and smoothed (4 mm full width at half maximum Gaussian kernel) using Statistical Parametric Mapping-5. Independent component analysis was then performed separately for each individual scanning session (one single session was acquired per subject), after removal of low-frequency drifts (150 s high-pass filter). We allowed FSL to use a probabilistic estimation of the number of components as implemented in the probabilistic independent component analysis MELODIC framework (Beckman et al., 2005; Beckman and Smith, 2005) aiming to identify the number of non-Gaussian sources in the data (and thus the optimal number of components needed to decompose the data), and attempting to avoid under- or over-fitting due to an incorrect number of sources. The best-fit component for each subject was then selected in an automated three-step process described as the ‘goodness-of-fit’ approach (Greicius et al., 2004, 2008; Seeley et al., 2007). This method allows for the unbiased selection of the component for each subject that best corresponds to the DMN. The template used in the present analysis for component selection was obtained from an independent dataset encompassing 19 healthy volunteers (age range 21–31 years) studied on another 3 T MRI scanner (see Supplementary Material for methodological details). First, because intrinsic connectivity is detected in the very low-frequency range (Cordes et al., 2001), a frequency filter was applied to remove any components in which high-frequency signal (>0.1 Hz) constituted 50% or more of the power in the Fourier spectrum. Next, we obtained goodness-of-fit scores to the DMN template for the remaining low-frequency components of each subject. To do this, the template-matching procedure calculated the average Z-score of voxels falling within the chosen template minus the average Z-score of voxels outside the template and selected the component in which this difference (the goodness-of-fit) is the greatest. Z-scores here reflect the degree to which the time series of a given voxel correlates with the time series corresponding to the specific independent component analysis component, scaled by the standard deviation of the error term. The Z-score is therefore a measure of how many standard deviations the signal is from the background noise. Finally, the component with the highest goodness-of-fit score was selected as the ‘best-fit’ component and used in the subsequent group analysis. This template-matching procedure was performed separately for each subject. It is important to note that this approach does not alter the components to fit the template in any way, but merely scores the pre-estimated components on how well they match the template (Seeley et al., 2007).

Next, all group analyses were performed on the subjects’ best-fit component Z-score images. We used a random-effects model, estimating the error variance across subjects (Holmes and Friston, 1998), consisting of an ANOVA with the four different states of consciousness (controls, minimally conscious state, vegetative state and coma patients) as the between subjects factor. A correction for non-sphericity was applied to account for potentially unequal variance across groups. A first analysis aimed at identifying the DMN in the control population. A second analysis searched for linear: Embedded Image non-linear (exponential): Embedded Image and power law: Embedded Image correlations between DMN connectivity and the level of consciousness (i.e. controls, minimally conscious state, vegetative state and coma). A third analysis looked for differences in DMN connectivity between minimally conscious state and unconscious (vegetative state and coma) patients, using a conjunction approach. A fourth analysis searched for a correlation between DMN connectivity and summed CRS-R scores. A supplementary multiple regression random effects analysis compared the single locked-in syndrome patient to other patient populations. In all our group level analyses, the subjects’ age was added as a confounding factor. Results in controls were thresholded at P < 0.05 corrected for false discovery rate at the whole brain level. All other analyses were thresholded at P < 0.05 corrected for false discovery rate in a 10 mm radius spherical small volume centred on a priori coordinates (all peak voxels from an independent DMN connectivity analysis in healthy controls previously published in Boly et al., 2009).

Finally, we computed the power spectrum of the time course of each DMN component and compared obtained peak frequencies (i.e. the frequency with maximum power). Two-tailed permutation tests (Nichols and Holmes, 2002) looked for group differences using EEGLAB (Delorme and Makeig, 2004) implemented in MATLAB 7 (Mathworks, Natick, MA, USA) and results were thresholded for significance at P < 0.05.


In controls, the DMN could be reproducibly identified as a set of areas encompassing posterior cingulate cortex (PCC) /precuneus, temporo-parietal junction, medial prefrontal cortex, para-hippocampal gyri, superior frontal sulci and thalamus (Fig. 1 and Table 2). The assessed non-linear functions showed a significant correlation between DMN connectivity strength and the level of consciousness in all the previously mentioned areas. Quasi identical results were obtained when exponential or power law contrasts were employed, whereas a less good fit was observed for the linear correlation, though linear correlation between connectivity and consciousness was significant (Table 3 and Fig. 2 show results for the exponential correlation). Clinical experience indicates that the decrease in consciousness between normal wakefulness, minimally conscious, vegetative state and coma is indeed non-linear (vegetative state patients’ consciousness being closer to comatose patients’ consciousness than to minimal consciousness). In all analyses, the peak area of significance for the correlation between connectivity and consciousness was found to be the PCC/precuneus. PCC/precuneus connectivity was also found to differentiate minimally conscious from unconscious patients (Table 4, Fig. 3). No brain area was found to be more present in DMN connectivity maps in unconscious compared with minimally conscious patients. No brain area could be identified as presenting a weaker connectivity in the single locked-in syndrome patient compared with controls. For illustrative purposes, the locked-in syndrome patient’s data are displayed as red circles in Fig. 2, allowing comparison to controls’ and patients’ data.

Figure 1

Default network identified in controls. Results are thresholded for display at whole brain false discovery rate corrected P < 0.01 and rendered on the mean T1 structural image of the controls.

Figure 2

Default network connectivity correlates with the level of consciousness, ranging from healthy controls, to minimally conscious, vegetative then comatose patients. (A) Areas showing a linear correlation between default network connectivity and consciousness. Results are thresholded for display at uncorrected P < 0.05 and rendered on the mean T1 structural image of the patients. (B) Mean Z-scores and 90% confidence interval for default network connectivity in PCC/precuneus, temporo-parietal junction, medial prefrontal cortex and parahippocampal gyrus across patient populations. Locked-in syndrome patient Z-scores are displayed for illustrative purposes as an additional red circles overlaid on control population data.

Figure 3

Brain areas within the default network connectivity which differentiate minimally conscious patients from unconscious patients. Results are thresholded for display at uncorrected P < 0.05 and rendered on the mean T1 structural image of the patients.

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Table 2

Peak voxels of the default network identified in healthy volunteers

Posterior cingulate cortex/precuneus−8−52286.82<0.001
Medial prefrontal cortex852325.53<0.001
Superior frontal sulcusR2824405.22<0.001
Temporo-parietal junctionR52−56245.41<0.001
Parahippocampal gyrusL−28−32−204.95<0.001
Temporal cortexR64−8−245.44<0.001
Inferior frontal gyrusR4024−204.040.001
Post-central gyrusL−52−16443.590.002
  • P-values are corrected for false discovery rate at the whole brain level.

View this table:
Table 3

Peak voxels showing an exponential correlation between default network connectivity and the level of consciousness

Posterior cingulate cortex/precuneus−8−52284.890.004
Medial prefrontal cortex052−203.520.025
Superior frontal sulcusR852354.620.005
Temporo-parietal junctionR60−64243.560.025
Parahippocampal gyrusL−32−20−243.830.014
Temporal cortexR56−12−244.290.007
Precentral gyrusL−48−20523.830.014
Postcentral gyrusL−52−16443.350.033
  • P-values are corrected for false discovery rate for whole brain.

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Table 4

Default network areas differentiating minimally conscious from unconscious (vegetative and coma) patients (conjunction approach)

Minimally conscious state>unconscious
Posterior cingulate cortex/precuneus20−48323.620.012
Medial prefrontal cortex−452−241.810.035*
Temporo-parietal junctionR64−52242.350.010*
Parahippocampal cortexL−32−20−242.110.017*
Temporal cortexR60−56282.350.010*
Minimally conscious state<unconscious
No areas could be identified
  • P-values are corrected for false discovery rate in a 10 mm radius spherical small volume centred on a priori coordinates. *Non-corrected P-values.

We observed a correlation between connectivity and CRS-R total scores in most of the DMN (Table 5), but results only survived correction for multiple comparisons in the medial prefrontal gyrus.

View this table:
Table 5

Peak voxels showing a correlation between default network connectivity and the CRS-R total score

Posterior cingulate cortex/precuneus8−32402.340.010*
Medial frontal gyrus−448363.780.006
Superior frontal sulcusR1632401.700.044*
Parahippocampal cortexL−40−4−241.860.032*
Temporo-parietal junctionR44−68481.890.030*
Temporal cortexR600402.360.009*
  • P-values are corrected for false discovery rate in a 10 mm radius spherical small volume centred on a priori coordinates. *Non-corrected P-values.

The total number of components and the proportion of variance explained by the DMN component were not significantly different in patients as compared with healthy controls [33 ± 20 (range 11–68) versus 27 ± 4 (range 21–35) components; and 4.1 ± 2.3 (range 1.2–8.7) versus 3.6 ± 0.8 (range 2.6–5.7), respectively]. Finally, the power spectrum of the DMN time courses showed a non-significant increase in peak frequency in patients as compared with healthy volunteers (mean ± SD, 0.040 ± 0.037 range 0.001–0.100 Hz versus 0.020 ± 0.013 range 0.001–0.056 Hz, respectively).


Clinical and neuroscientific relevance of a correlation between DMN connectivity and the level of consciousness

Using resting state fMRI connectivity analyses, we showed an exponential correlation between DMN connectivity integrity and the level of consciousness of brain-damaged patients ranging from controls, to minimally conscious, vegetative state then coma patients. These results suggest that, although the DMN can still be identified in unconscious patients, as in anaesthetized monkeys (Vincent et al., 2007), connectivity strength within DMN could possibly be a reliable indicator of a patient’s level of consciousness, differentiating unconscious patients such as those in a coma or vegetative state from minimally conscious and locked-in syndrome patients. As resting state fMRI studies are much easier to acquire in a routine clinical setting than standard fMRI paradigms, these connectivity studies could potentially be a useful complement to bedside behavioural assessment in the evaluation of the level of consciousness of non-communicative brain-damaged patients. Note that CRS-R total scores showed a less significant fit as compared with the non-linear correlation with DMN connectivity. In our view, this is explained by the fact that the CRS-R total score was not developed to differentiate between different levels of consciousness (e.g. a minimally conscious patient may have an identical CRS-R total score as a vegetative state patient).

In addition to its potential clinical relevance, the finding that DMN connectivity strength is proportional to the level of consciousness of brain-damaged patients sheds light on the significance of spontaneous brain activity fluctuations as recorded by fMRI. Our results suggest that the strength of connectivity in resting state fMRI-identified networks could be related in a quantitative manner to the level of conscious processing in severely brain-damaged patients. These results complement previous findings showing partially preserved connectivity in states of altered consciousness like vegetative state (Boly et al., 2009), light sleep (Horovitz et al., 2008) or anaesthetized monkey (Vincent et al., 2007). Our results are also in line with recent reports of decreased DMN connectivity in healthy volunteers during sedation (Greicius et al., 2008) and deep sleep (Horovitz et al., 2009). Larson-Prior et al. (2009), however, observed no measurable change in DMN connectivity during light sleep. Taken together, these findings suggest a two-layer view of resting state fMRI DMN connectivity: one part of the DMN connectivity would persist independently of the level of consciousness, and possibly related to underlying anatomical connectivity (Vincent et al., 2007; Greicius et al., 2009), and the other part being more tightly related to the presence of conscious cognitive processes. More generally, the finding of decreased DMN connectivity in proportion to impairment of consciousness is consistent with previous findings of metabolic impairment of a large frontoparietal network, encompassing main nodes of the DMN, in patients in vegetative state compared with controls, as well as in other states of clinical unconsciousness such as sleep, anaesthesia, seizures or somnambulism (Baars et al., 2003; Laureys, 2005). The current results are also consistent with the so-called ‘global workspace’ theory of consciousness (Baars et al., 2003; Dehaene and Changeux, 2005), by suggesting that higher order frontoparietal areas are likely to play a crucial role in the genesis of conscious perception. However, we should remain cautious when interpreting the functional significance of DMN connectivity measurements in terms of consciousness. More investigations on physiological (e.g. sleep and hypnotic state), pharmacological (e.g. general anaesthesia) and pathological alterations of consciousness in health and disease subjects, are awaited before a consensus can be reached on the precise functional meaning of this network.

The maximum peak of significance for a correlation between DMN connectivity and consciousness was found in the PCC/precuneus. This finding is coherent with studies showing a central role of precuneus in DMN architecture, from the functional (Fransson and Marrelec, 2008) to the structural point of view (Hagmann et al., 2008). Precuneus connectivity could also reliably differentiate minimally conscious from unconscious patients, again suggesting a particularly strong relationship between the level of activity of this area and the patients’ level of consciousness (Laureys et al., 2004; Cavanna and Trimble, 2006). As the DMN has been suggested to be involved in ‘mind-wandering’ (Mason et al., 2007) and self-referential processes (Cavanna and Trimble, 2006; Cavanna, 2007), our results could also imply that minimally conscious patients have a partially preserved level of self-awareness or ‘day-dreaming’-like cognition or, at a minimum, have the residual functional architecture to support such complex processes. This finding stresses the importance of assessing residual cognitive functions in patients with disorders of consciousness, which could be largely underestimated at the clinical bedside.

It should be noted that while the thalamus has not been reported in all DMN connectivity studies in the literature, it has been described in papers on healthy volunteers in recent years (Fransson, 2005; De Luca et al., 2006; Greicius et al., 2007; Boly et al., 2009). Thalamo-cortical loops have been increasingly associated with conscious perception (Laureys et al., 2000; White and Alkire, 2003; Schiff et al., 2007), possibly explaining our observed correlation between consciousness and thalamo-cortical DMN connectivity.

Technical issues in the study of resting state fMRI connectivity in severely brain-damaged patients

Several technical issues should be discussed in the study of connectivity in patients with altered states of consciousness. While the acquisition of resting state fMRI data is easier than standard fMRI paradigms, spontaneous blood oxygen level dependent fluctuations measures are subject to more artefactual bias than evoked data (Morcom and Fletcher, 2007). Therefore, resting state fMRI analyses should include a method for carefully separating relevant signal from artefacts present in the data. Independent component analysis is especially suited to this aim. Compared with region of interest-driven correlation analyses, independent component analysis offers the double advantage of being able to isolate cortical connectivity maps from non-neural signals (Beckmann et al., 2005; Beckman and Smith, 2005; Seeley et al., 2007), and of being unbiased by the selection of a seed region-of-interest for correlation analysis. Therefore, independent component analysis may allow the identification of network nodes missed by the conventional region of interest-driven analysis (Seeley et al., 2007). The selection of relevant components of interest is still an issue when dealing with the outputs of independent component analysis. In a clinical framework, the use of an automated component selection approach, as used in the present analysis, is ideally required, in order to avoid subjective bias in the interpretation of data. In this study, the template used for automatic DMN component selection was issued from an independent connectivity study on a separate group of healthy volunteers, and was applied to both controls and patients. It should be noted that this methodology, classically employed in clinical studies on DMN connectivity [e.g. in dementia (Greicius et al., 2004), depression (Greicius et al., 2007) or epileptic patients (Zhang et al., 2009)], might bias the selection towards the healthy control group. Future work should compare the presently used ‘goodness-of-fit’ approach to other component selection approaches based on spatial similarity with templates, or ‘fingerprinting’ approaches [i.e. graphical representations of independent components in multidimensional space encompassing spatial and temporal entropy, kurtosis, one-lag auto-correlation and power contributions in different frequency bands (De Martino et al., 2007)] in order to test for reliability at the individual level. Another remaining question is the relationship between structural and functional connectivity changes in non-communicative brain-damaged patients. Functional connectivity in DMN has indeed been related to underlying structural anatomy (Greicius et al., 2009). Further multimodal studies should combine resting state functional MRI data with structural MRI (e.g. diffusion tensor imaging data) or high-density EEG recordings.


We here identified a significant correlation, at the group-level, between DMN connectivity and the level of consciousness. These results suggest that after further validation, the present methodology could potentially be rapidly translated into a routine clinical setting and bring relevant ancillary information on a patient’s residual brain function to bear on their clinical evaluation. The presence of an exponential correlation between DMN connectivity and consciousness suggests that resting state fMRI could be a potentially useful paraclinical marker of the level of consciousness in non-communicative brain-damaged patients, complementing their bedside assessment. Future studies on larger samples of patients will aim at correlating resting state fMRI connectivity with prognosis and white matter damage (as assessed, for example, by diffusion tensor imaging) in individual brain-damaged patients.


Belgian Fonds National de la Recherche Scientifique (FNRS); European Commission (Mindbridge, DISCOS, CATIA and DECODER); Mind Science Foundation; James McDonnell Foundation; French Speaking Community Concerted Research Action (ARC 06/11-340); Fondation Médicale Reine Elisabeth; NIH (NS048302 to MD.G.).

Supplementary material

Supplementary material is available at Brain online.


The authors thank the technicians of the Department of Neuro-Radiology and the nurses of the Intensive Care and Neurology Departments of the Centre Hospitalier Universitaire of Liège for their active participation in the MRI studies in comatose patients. M.-A.B. is Research Fellow, M.B., Q.N. and C.S. are Post-doctoral Fellows, and S.L. and P.M. are Senior Research Associates at the FNRS, AV is Research Fellow at the ARC 06/11-340.


  • *These authors contributed equally to the work.

  • Abbreviations:
    Coma Recovery Scale-Revised
    default mode network
    functional magnetic resonance imaging
    posterior cingulate cortex


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