Colocalization of organelles with mutant androgen receptor (AR) accumulation. Double immunofluorescence staining with antibodies against TGN46 and expanded polyQ reveals that TGN46 and mutant AR are colocalized as shown for expanded poly Q, TGN46 and superimposition of the two signals in neurons of the spinal dorsal root ganglia in SBMA, suggesting that mutant AR exists in the Golgi apparatus. Cytoplasmic colocalization of cathepsin B, GRP78 and mitochondria with mutant AR is not observed in dorsal root ganglia (shown here) suggesting that the endoplasmic reticulum, lysosomes and mitochondria are unassociated with mutant AR. See Adachi et al., pp. 659-670.
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